4YZV

Precleavage 70S structure of the P. vulgaris HigB deltaH92 toxin bound to the ACA codon

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, DB (QA, XA)	16S rRNA	polymer	1522	493958.3	2	GenBank (55771382)	Thermus thermophilus HB8
2	B, EB (QB, XB)	30S ribosomal protein S2	polymer	256	29317.7	2	UniProt (P80371) Pfam (PF00318)	Thermus thermophilus HB8
3	C, FB (QC, XC)	30S ribosomal protein S3	polymer	239	26751.1	2	UniProt (P80372) Pfam (PF07650) Pfam (PF00189)	Thermus thermophilus HB8
4	D, GB (QD, XD)	30S ribosomal protein S4	polymer	209	24373.4	2	UniProt (P80373) Pfam (PF00163) Pfam (PF01479)	Thermus thermophilus HB8
5	E, HB (QE, XE)	30S ribosomal protein S5	polymer	162	17583.4	2	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719)	Thermus thermophilus HB8
6	F, IB (QF, XF)	30S ribosomal protein S6	polymer	101	11988.8	2	UniProt (Q5SLP8) Pfam (PF01250)	Thermus thermophilus HB8	TS9
7	G, JB (QG, XG)	30S ribosomal protein S7	polymer	156	18051.0	2	UniProt (P17291) Pfam (PF00177)	Thermus thermophilus HB8
8	H, KB (QH, XH)	30S ribosomal protein S8	polymer	138	15868.6	2	UniProt (Q5SHQ2)	Thermus thermophilus HB8
9	I, LB (QI, XI)	30S ribosomal protein S9	polymer	128	14410.6	2	UniProt (P80374) Pfam (PF00380)	Thermus thermophilus HB8
10	J, MB (QJ, XJ)	30S ribosomal protein S10	polymer	105	11955.0	2	UniProt (Q5SHN7) Pfam (PF00338)	Thermus thermophilus HB8
11	K, NB (QK, XK)	30S ribosomal protein S11	polymer	129	13737.9	2	UniProt (P80376) Pfam (PF00411)	Thermus thermophilus HB8
12	L, OB (QL, XL)	30S ribosomal protein S12	polymer	132	14637.4	2	UniProt (Q5SHN3) Pfam (PF00164)	Thermus thermophilus HB8
13	M, PB (QM, XM)	30S ribosomal protein S13	polymer	126	14338.9	2	UniProt (P80377) Pfam (PF00416)	Thermus thermophilus HB8
14	N, QB (QN, XN)	30S ribosomal protein S14 type Z	polymer	61	7158.7	2	UniProt (Q5SHQ1)	Thermus thermophilus HB8
15	O, RB (QO, XO)	30S ribosomal protein S15	polymer	89	10578.4	2	UniProt (Q5SJ76) Pfam (PF00312)	Thermus thermophilus HB8
16	P, SB (QP, XP)	30S ribosomal protein S16	polymer	88	10410.0	2	UniProt (Q5SJH3) Pfam (PF00886)	Thermus thermophilus HB8
17	Q, TB (QQ, XQ)	30S ribosomal protein S17	polymer	105	12325.7	2	UniProt (Q5SHP7)	Thermus thermophilus HB8
18	R, UB (QR, XR)	30S ribosomal protein S18	polymer	88	10258.3	2	UniProt (Q5SLQ0) Pfam (PF01084)	Thermus thermophilus HB8
19	S, VB (QS, XS)	30S ribosomal protein S19	polymer	93	10605.5	2	UniProt (Q5SHP2) Pfam (PF00203)	Thermus thermophilus HB8
20	T, WB (QT, XT)	30S ribosomal protein S20	polymer	106	11736.1	2	UniProt (P80380) Pfam (PF01649)	Thermus thermophilus HB8
21	U, XB (QU, XU)	30S ribosomal protein Thx	polymer	25	3089.7	2	UniProt (Q5SIH3) Pfam (PF17070)	Thermus thermophilus HB8	S31
22	V, YB (QV, XV)	P-site tRNA-fMet	polymer	77	24802.8	2	GenBank (751399318)	Escherichia coli
23	W, ZB (QX, XX)	messenger RNA	polymer	24	7846.8	2		Escherichia coli
24	AC, X (QY, XY)	Killer protein	polymer	117	13575.3	2	UniProt (Q7A225) Pfam (PF05015)	Proteus vulgaris
25	BC, Y (RA, YA)	23S rRNA	polymer	2916	948240.6	2	GenBank (55771382)	Thermus thermophilus HB8
26	CC, Z (RB, YB)	5S rRNA	polymer	124	40152.9	2	GenBank (55771382)	Thermus thermophilus HB8
27	AA, DC (RD, YD)	50S ribosomal protein L2	polymer	276	30532.6	2	UniProt (P60405) Pfam (PF00181) Pfam (PF03947)	Thermus thermophilus HB8
28	BA, EC (RE, YE)	50S ribosomal protein L3	polymer	206	22450.2	2	UniProt (Q5SHN8) Pfam (PF00297)	Thermus thermophilus HB8
29	CA, FC (RF, YF)	50S ribosomal protein L4	polymer	210	23271.9	2	UniProt (Q5SHN9) Pfam (PF00573)	Thermus thermophilus HB8	L1e
30	DA, GC (RG, YG)	50S ribosomal protein L5	polymer	182	21061.6	2	UniProt (Q5SHQ0) Pfam (PF00281) Pfam (PF00673)	Thermus thermophilus HB8
31	EA, HC (RH, YH)	50S ribosomal protein L6	polymer	180	19568.9	2	UniProt (Q5SHQ3)	Thermus thermophilus HB8
32	FA, IC (RI, YI)	50S ribosomal protein L9	polymer	148	16422.2	2	UniProt (Q5SLQ1) Pfam (PF01281) Pfam (PF03948)	Thermus thermophilus HB8
33	GA, JC (RN, YN)	50S ribosomal protein L13	polymer	140	15927.9	2	UniProt (P60488) Pfam (PF00572)	Thermus thermophilus HB8
34	HA, KC (RO, YO)	50S ribosomal protein L14	polymer	122	13323.6	2	UniProt (Q5SHP8) Pfam (PF00238)	Thermus thermophilus HB8
35	IA, LC (RP, YP)	50S ribosomal protein L15	polymer	150	16319.1	2	UniProt (Q5SHQ7) Pfam (PF00828)	Thermus thermophilus HB8
36	JA, MC (RQ, YQ)	50S ribosomal protein L16	polymer	141	15993.9	2	UniProt (P60489) Pfam (PF00252)	Thermus thermophilus HB8
37	KA, NC (RR, YR)	50S ribosomal protein L17	polymer	118	13750.1	2	UniProt (Q9Z9H5) Pfam (PF01196)	Thermus thermophilus HB8
38	LA, OC (RS, YS)	50S ribosomal protein L18	polymer	112	12639.8	2	UniProt (Q5SHQ4) Pfam (PF00861)	Thermus thermophilus HB8	TL24
39	MA, PC (RT, YT)	50S ribosomal protein L19	polymer	146	17188.8	2	UniProt (P60490) Pfam (PF01245)	Thermus thermophilus HB8
40	NA, QC (RU, YU)	50S ribosomal protein L20	polymer	118	13779.3	2	UniProt (P60491) Pfam (PF00453)	Thermus thermophilus HB8
41	OA, RC (RV, YV)	50S ribosomal protein L21	polymer	101	11069.2	2	UniProt (P60492) Pfam (PF00829)	Thermus thermophilus HB8
42	PA, SC (RW, YW)	50S ribosomal protein L22	polymer	113	12808.1	2	UniProt (Q5SHP3) Pfam (PF00237)	Thermus thermophilus HB8
43	QA, TC (RX, YX)	50S ribosomal protein L23	polymer	96	10759.8	2	UniProt (Q5SHP0) Pfam (PF00276)	Thermus thermophilus HB8
44	RA, UC (RY, YY)	50S ribosomal protein L24	polymer	110	12085.6	2	UniProt (Q5SHP9) Pfam (PF00467) Pfam (PF17136)	Thermus thermophilus HB8
45	SA, VC (RZ, YZ)	50S ribosomal protein L25	polymer	206	23238.6	2	UniProt (Q5SHZ1) Pfam (PF01386) Pfam (PF14693)	Thermus thermophilus HB8	TL5
46	TA, WC (R0, Y0)	50S ribosomal protein L27	polymer	85	9529.1	2	UniProt (P60493) Pfam (PF01016)	Thermus thermophilus HB8
47	UA, XC (R1, Y1)	50S ribosomal protein L28	polymer	98	11004.2	2	UniProt (P60494) Pfam (PF00830)	Thermus thermophilus HB8
48	VA, YC (R2, Y2)	50S ribosomal protein L29	polymer	72	8670.3	2	UniProt (Q5SHP6) Pfam (PF00831)	Thermus thermophilus HB8
49	WA, ZC (R3, Y3)	50S ribosomal protein L30	polymer	60	6799.1	2	UniProt (Q5SHQ6) Pfam (PF00327)	Thermus thermophilus HB8
50	AD, XA (R4, Y4)	50S ribosomal protein L31	polymer	71	8300.5	2	UniProt (Q5SJE1) Pfam (PF01197)	Thermus thermophilus HB8
51	BD, YA (R5, Y5)	50S ribosomal protein L32	polymer	60	6722.1	2	UniProt (P80339) Pfam (PF01783)	Thermus thermophilus HB8
52	CD, ZA (R6, Y6)	50S ribosomal protein L33	polymer	54	6632.9	2	UniProt (P35871) Pfam (PF00471)	Thermus thermophilus HB8
53	AB, DD (R7, Y7)	50S ribosomal protein L34	polymer	49	6132.4	2	UniProt (P80340) Pfam (PF00468)	Thermus thermophilus HB8
54	BB, ED (R8, Y8)	50S ribosomal protein L35	polymer	65	7506.2	2	UniProt (Q5SKU1) Pfam (PF01632)	Thermus thermophilus HB8
55	CB, FD (R9, Y9)	50S ribosomal protein L36	polymer	37	4435.4	2	UniProt (Q5SHR2) Pfam (PF00444)	Thermus thermophilus HB8	Ribosomal protein B
56	GD, HD (Z6, Z7)	CC-puromycin	polymer	3	1098.9	2		Streptomyces alboniger
57	AAA, AAB, ABA, ABB, ACA... (RA, YA, RB, XA, Y0...)	MAGNESIUM ION	non-polymer		24.3	1346	Chemie (MG)
58	DJ, FIA, IJ, LIA (QD, XD, QN, XN)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

QY, XY: 2 - 91 (UniProt: Q7A225)

PDB	External Database	Details
Met 0	-	initiating methionine
Gly 1	-	expression tag
Lys 92	-	expression tag
Leu 93	-	expression tag
Gly 94	-	expression tag
Pro 95	-	expression tag
Glu 96	-	expression tag
Gln 97	-	expression tag
Lys 98	-	expression tag
Leu 99	-	expression tag
Ile 100	-	expression tag
Ser 101	-	expression tag
Glu 102	-	expression tag
Glu 103	-	expression tag
Asp 104	-	expression tag
Leu 105	-	expression tag
Asn 106	-	expression tag
Ser 107	-	expression tag
Ala 108	-	expression tag
Val 109	-	expression tag
Asp 110	-	expression tag
His 111	-	expression tag
His 112	-	expression tag
His 113	-	expression tag
His 114	-	expression tag
His 115	-	expression tag
His 116	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	112
	Total formula weight	4433549.9
Non-Polymers*	Number of molecules	1350
	Total formula weight	32976.2
All*	Total formula weight	4466526.1

*Water molecules are not included.