4YX7

Complex of SpaO(SPOA1,2) and OrgB(APAR)::T4lysozyme fusion protein

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D	Surface presentation of antigens protein SpaO	polymer	73	8246.6	2	UniProt (P40699) In PDB	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
2	B, E	Surface presentation of antigens protein SpaO	polymer	70	7710.8	2	UniProt (P40699) Pfam (PF01052) In PDB	Salmonella typhimurium
3	C, F	Oxygen-regulated invasion protein OrgB,Endolysin	polymer	197	22162.6	2	UniProt (P0CL45) UniProt (P00720) Pfam (PF00959) In PDB	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)	Lysis protein,Lysozyme,Muramidase
4		water	water		18.0	657	Chemie (HOH)

Sequence modifications

A, D: 5 - 73 (UniProt: P40699)

PDB	External Database	Details
Gly 1	-	expression tag
Pro 2	-	expression tag
Val 3	-	expression tag
Asp 4	-	expression tag

B, E: 5 - 70 (UniProt: P40699)

PDB	External Database	Details
Gly 1	-	expression tag
Pro 2	-	expression tag
Val 3	-	expression tag
Asp 4	-	expression tag

C, F: 5 - 34 (UniProt: P0CL45)

PDB	External Database	Details
Gly 1	-	expression tag
Pro 2	-	expression tag
Val 3	-	expression tag
Asp 4	-	expression tag

C, F: 35 - 197 (UniProt: P00720)

PDB	External Database	Details
Gly 45	Arg 12	conflict
Asn 53	Asp 20	engineered mutation
Thr 87	Cys 54	engineered mutation
Ala 130	Cys 97	engineered mutation
Arg 170	Ile 137	conflict
Ala 195	Lys 162	engineered mutation
Ala 196	Asn 163	engineered mutation
Ala 197	Leu 164	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	76239.9
All*	Total formula weight	76239.9

*Water molecules are not included.