4YTR

Crystal structure of D-tagatose 3-epimerase C66S from Pseudomonas cichorii in complex with 1-deoxy L-tagatose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	D-tagatose 3-epimerase	polymer	300	33858.6	4	UniProt (O50580) Pfam (PF01261)	Pseudomonas cichorii
2	E, G, J, O (A, B, C, D)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
3	F, H, I, L, M... (B, C, D)	1-deoxy-beta-L-tagatopyranose	non-polymer		164.2	9	Chemie (TGK)
4	K (C)	1-deoxy-L-tagatose	non-polymer		164.2	1	Chemie (TGJ)
5	S, T, U, V (A, B, C, D)	water	water		18.0	1151	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 290 (UniProt: O50580)

PDB	External Database	Details
Ser 66	Cys 66	engineered mutation
Gly 291	-	expression tag
Ser 292	-	expression tag
Arg 293	-	expression tag
Ser 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	135434.5
Non-Polymers*	Number of molecules	14
	Total formula weight	1861.3
All*	Total formula weight	137295.8

*Water molecules are not included.