4YR1
Crystal Structure of E. Coli Alkaline Phosphatase D101A/D153A in complex with inorganic phosphate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Alkaline phosphatase | polymer | 443 | 46267.5 | 2 | UniProt (P00634) Pfam (PF00245) | Escherichia coli (strain K12) | APase |
| 2 | C, D, I, J (A, B) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 3 | E, K (A, B) | PHOSPHATE ION | non-polymer | 95.0 | 2 | Chemie (PO4) | |||
| 4 | F, G, H, L (A, B) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 5 | M, N (A, B) | water | water | 18.0 | 182 | Chemie (HOH) |
Sequence modifications
A, B: 9 - 449 (UniProt: P00634)
| PDB | External Database | Details |
|---|---|---|
| Ala 101 | Asp 123 | engineered mutation |
| Ala 153 | Asp 175 | engineered mutation |
| Ser 450 | - | expression tag |
| Ala 451 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 92535.0 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 820.0 | |
| All* | Total formula weight | 93354.9 |
