4YIS
Crystal Structure of LAGLIDADG Meganuclease I-CpaMI Bound to Uncleaved DNA
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Meganuclease I-CpaMI | polymer | 295 | 33652.5 | 2 | UniProt (O20960) Pfam (PF00961) | Cryphonectria parasitica (Chesnut blight fungus) | |
| 2 | C, E (E, C) | DNA (28-MER) | polymer | 28 | 8439.5 | 2 | synthetic construct | ||
| 3 | D, F (F, D) | DNA (28-MER) | polymer | 28 | 8747.6 | 2 | synthetic construct | ||
| 4 | G, H, J, K, L (A, B) | CALCIUM ION | non-polymer | 40.1 | 5 | Chemie (CA) | |||
| 5 | I, M, N, O, P (A, B, E, F, D) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 5 | Chemie (EDO) | |||
| 6 | Q, R, S, T, U... (A, B, E, F, C...) | water | water | 18.0 | 37 | Chemie (HOH) |
Sequence modifications
A, B: 5 - 299 (UniProt: O20960)
| PDB | External Database | Details |
|---|---|---|
| Asn 50 | Leu 167 | conflict |
| Gln 100 | Trp 217 | conflict |
| Lys 114 | Leu 231 | conflict |
| Asn 115 | Leu 232 | conflict |
| Asn 161 | Phe 278 | conflict |
| Gln 264 | Leu 381 | conflict |
| Gln 267 | Ile 384 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 101679.2 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 510.7 | |
| All* | Total formula weight | 102189.9 |
