4YIR

Crystal structure of Rad4-Rad23 crosslinked to an undamaged DNA

Replaces:  4U29

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	DNA repair protein RAD4	polymer	538	62588.5	1	UniProt (P14736) Pfam (PF03835) Pfam (PF10403) Pfam (PF10405)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
2	B (X)	UV excision repair protein RAD23	polymer	171	17783.4	1	UniProt (P32628) Pfam (PF09280) Pfam (PF00627)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
3	C (W)	DNA (5'-D(*TP*TP*GP*AP*CP*TP*CP*(G47)P*AP*CP*AP*TP*CP*CP*CP*CP*CP*GP*CP*TP*AP*CP*AP*A)-3')	polymer	24	7294.8	1		synthetic construct
4	D (Y)	DNA (5'-D(*AP*TP*TP*GP*TP*AP*GP*CP*G*GP*GP*GP*AP*TP*GP*TP*CP*GP*AP*GP*TP*CP*A)-3')	polymer	24	7505.8	1		synthetic construct

Sequence modifications

A: 101 - 632 (UniProt: P14736)

PDB	External Database	Details
Gly 95	-	expression tag
Ser 96	-	expression tag
Ser 97	-	expression tag
Arg 98	-	expression tag
Ala 99	-	expression tag
Met 100	-	expression tag
Thr 115	Lys 115	conflict
Cys 131	Val 131	engineered mutation
Ser 132	Cys 132	engineered mutation
Glu 223	Val 223	conflict
Arg 427	Gln 427	conflict

X: 230 - 398 (UniProt: P32628)

PDB	External Database	Details
Gly 228	-	expression tag
Ser 229	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	95172.5
All*	Total formula weight	95172.5

*Water molecules are not included.