4YGB

Crystal structure of ERGIC-53/MCFD2, monoclinic calcium-free form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C (A, C)	Protein ERGIC-53	polymer	246	27154.2	2	UniProt (P49257) Pfam (PF03388)	Homo sapiens (Human)	ER-Golgi intermediate compartment 53 kDa protein,Gp58,Intracellular mannose-specific lectin MR60,Lectin mannose-binding 1
2	B, D (B, D)	Multiple coagulation factor deficiency protein 2	polymer	104	12056.2	2	UniProt (Q8NI22) Pfam (PF13499)	Homo sapiens (Human)	Neural stem cell-derived neuronal survival protein
3	E, H (A, C)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
4	F, G, I, J (B, D)	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
5	K, L, M, N (A, B, C, D)	water	water		18.0	239	Chemie (HOH)

Sequence modifications

A, C: 31 - 269 (UniProt: P49257)

PDB	External Database	Details
Met 24	-	expression tag
Asn 25	-	expression tag
His 26	-	expression tag
Lys 27	-	expression tag
Val 28	-	expression tag
His 29	-	expression tag
Met 30	-	expression tag

B, D: 67 - 146 (UniProt: Q8NI22)

PDB	External Database	Details
Met 43	-	expression tag
Gly 44	-	expression tag
His 45	-	expression tag
His 46	-	expression tag
His 47	-	expression tag
His 48	-	expression tag
His 49	-	expression tag
His 50	-	expression tag
His 51	-	expression tag
His 52	-	expression tag
His 53	-	expression tag
His 54	-	expression tag
Ser 55	-	expression tag
Ser 56	-	expression tag
Gly 57	-	expression tag
His 58	-	expression tag
Ile 59	-	expression tag
Glu 60	-	expression tag
Gly 61	-	expression tag
Arg 62	-	expression tag
His 63	-	expression tag
Met 64	-	expression tag
Leu 65	-	expression tag
Glu 66	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	78420.8
Non-Polymers*	Number of molecules	6
	Total formula weight	344.5
All*	Total formula weight	78765.3

*Water molecules are not included.