4YDU

Crystal structure of E. coli YgjD-YeaZ heterodimer in complex with ADP

Replaces:  4WOS

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	tRNA N6-adenosine threonylcarbamoyltransferase	polymer	343	36879.2	2	UniProt (P05852) Pfam (PF00814)	Escherichia coli	YgjD,N6-L-threonylcarbamoyladenine synthase,t(6)A synthase,t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaD,tRNA threonylcarbamoyladenosine biosynthesis protein TsaD
2	C, D (C, D)	tRNA threonylcarbamoyladenosine biosynthesis protein TsaB	polymer	237	26033.6	2	UniProt (P76256) Pfam (PF00814)	Escherichia coli	YeaZ,t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaB
3	E, L, Q, T (A, B, C, D)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)
4	F, M (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
5	G, N (A, B)	FE (III) ION	non-polymer		55.8	2	Chemie (FE)
6	H, I, J, K, O... (A, B, C, D)	ACETATE ION	non-polymer		59.0	13	Chemie (ACT)
7	AA, BA, CA, Z (B, C, D, A)	water	water		18.0	438	Chemie (HOH)

Sequence modifications

A, B: 1 - 337 (UniProt: P05852)

PDB	External Database	Details
His 338	-	expression tag
His 339	-	expression tag
His 340	-	expression tag
His 341	-	expression tag
His 342	-	expression tag
His 343	-	expression tag

C, D: 1 - 231 (UniProt: P76256)

PDB	External Database	Details
His 232	-	expression tag
His 233	-	expression tag
His 234	-	expression tag
His 235	-	expression tag
His 236	-	expression tag
His 237	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	125825.7
Non-Polymers*	Number of molecules	21
	Total formula weight	2636.7
All*	Total formula weight	128462.4

*Water molecules are not included.