4Y7E
Crystal structure of beta-mannanase from Streptomyces thermolilacinus with mannohexaose
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Endoglucanase | polymer | 346 | 37337.4 | 2 | UniProt (F5HR99) Pfam (PF00150) | Streptomyces thermolilacinus | |
| 2 | C (C) | beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose | branched | 1153.0 | 1 | In PDB GlyTouCan (G77805VI) | |||
| 3 | D (D) | beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose | branched | 504.4 | 1 | In PDB GlyTouCan (G26320VF) | |||
| 4 | E, F, P, Q, R... (A, B) | GLYCEROL | non-polymer | 92.1 | 7 | Chemie (GOL) | |||
| 5 | G, H, I, J, K... (A) | beta-D-mannopyranose | non-polymer | 180.2 | 7 | Chemie (BMA) | |||
| 6 | N, O, U, V, W (A, B) | CALCIUM ION | non-polymer | 40.1 | 5 | Chemie (CA) | |||
| 7 | X, Y (A, B) | water | water | 18.0 | 795 | Chemie (HOH) |
Sequence modifications
A, B: 36 - 349 (UniProt: F5HR99)
| PDB | External Database | Details |
|---|---|---|
| Gly 16 | - | expression tag |
| Ser 17 | - | expression tag |
| Ser 18 | - | expression tag |
| His 19 | - | expression tag |
| His 20 | - | expression tag |
| His 21 | - | expression tag |
| His 22 | - | expression tag |
| His 23 | - | expression tag |
| His 24 | - | expression tag |
| Ser 25 | - | expression tag |
| Ser 26 | - | expression tag |
| Gly 27 | - | expression tag |
| Leu 28 | - | expression tag |
| Val 29 | - | expression tag |
| Pro 30 | - | expression tag |
| Arg 31 | - | expression tag |
| Gly 32 | - | expression tag |
| Ser 33 | - | expression tag |
| His 34 | - | expression tag |
| Met 35 | - | expression tag |
| Ala 273 | Glu 273 | engineered mutation |
| Lys 350 | - | expression tag |
| Leu 351 | - | expression tag |
| Ala 352 | - | expression tag |
| Ala 353 | - | expression tag |
| Leu 354 | - | expression tag |
| Glu 355 | - | expression tag |
| His 356 | - | expression tag |
| His 357 | - | expression tag |
| His 358 | - | expression tag |
| His 359 | - | expression tag |
| His 360 | - | expression tag |
| His 361 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 74674.8 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1657.4 | |
| Non-Polymers* | Number of molecules | 19 |
| Total formula weight | 2106.1 | |
| All* | Total formula weight | 78438.4 |
