4Y7E
Crystal structure of beta-mannanase from Streptomyces thermolilacinus with mannohexaose
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Endoglucanase | polymer | 346 | 37337.4 | 2 | UniProt (F5HR99) Pfam (PF00150) In PDB | Streptomyces thermolilacinus | |
2 | C | beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose | branched | 1153.0 | 1 | In PDB GlyTouCan (G77805VI) | |||
3 | D | beta-D-mannopyranose-(1-4)-beta-D-mannopyranose-(1-4)-beta-D-mannopyranose | branched | 504.4 | 1 | In PDB GlyTouCan (G26320VF) | |||
4 | A, B | GLYCEROL | non-polymer | 92.1 | 7 | Chemie (GOL) | |||
5 | A | beta-D-mannopyranose | non-polymer | 180.2 | 7 | Chemie (BMA) | |||
6 | A, B | CALCIUM ION | non-polymer | 40.1 | 5 | Chemie (CA) | |||
7 | water | water | 18.0 | 795 | Chemie (HOH) |
Sequence modifications
A, B: 36 - 349 (UniProt: F5HR99)
PDB | External Database | Details |
---|---|---|
Gly 16 | - | expression tag |
Ser 17 | - | expression tag |
Ser 18 | - | expression tag |
His 19 | - | expression tag |
His 20 | - | expression tag |
His 21 | - | expression tag |
His 22 | - | expression tag |
His 23 | - | expression tag |
His 24 | - | expression tag |
Ser 25 | - | expression tag |
Ser 26 | - | expression tag |
Gly 27 | - | expression tag |
Leu 28 | - | expression tag |
Val 29 | - | expression tag |
Pro 30 | - | expression tag |
Arg 31 | - | expression tag |
Gly 32 | - | expression tag |
Ser 33 | - | expression tag |
His 34 | - | expression tag |
Met 35 | - | expression tag |
Ala 273 | Glu 273 | engineered mutation |
Lys 350 | - | expression tag |
Leu 351 | - | expression tag |
Ala 352 | - | expression tag |
Ala 353 | - | expression tag |
Leu 354 | - | expression tag |
Glu 355 | - | expression tag |
His 356 | - | expression tag |
His 357 | - | expression tag |
His 358 | - | expression tag |
His 359 | - | expression tag |
His 360 | - | expression tag |
His 361 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 74674.8 | |
Branched | Number of molecules | 2 |
Total formula weight | 1657.4 | |
Non-Polymers* | Number of molecules | 19 |
Total formula weight | 2106.1 | |
All* | Total formula weight | 78438.4 |