4Y2O

Structure of CFA/I pili chaperone-major subunit complex (CfaA-CfaB)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CfA/I fimbrial subunit A (Colonization factor antigen subunit A putative chaperone)	polymer	231	26493.5	1	UniProt (E3PPC3)	Escherichia coli O78:H11 (strain H10407 / ETEC)
2	B (B)	CFA/I fimbrial subunit B	polymer	142	14715.6	1	UniProt (E3PPC4) Pfam (PF04449)	Escherichia coli O78:H11 (strain H10407 / ETEC)	CFA/I antigen,CFA/I pilin,Colonization factor antigen I subunit B
3	C, D (A)	TRIETHYLENE GLYCOL	non-polymer		150.2	2	Chemie (PGE)
4	E, F (B)	NICKEL (II) ION	non-polymer		58.7	2	Chemie (NI)
5	G, H (A, B)	water	water		18.0	139	Chemie (HOH)

Sequence modifications

A: 0 - 219 (UniProt: E3PPC3)

PDB	External Database	Details
Met -1	-	initiating methionine
Ile 112	Thr 131	engineered mutation
Ala 220	-	expression tag
Ala 221	-	expression tag
Trp 222	-	expression tag
Ser 223	-	expression tag
His 224	-	expression tag
Pro 225	-	expression tag
Gln 226	-	expression tag
Phe 227	-	expression tag
Glu 228	-	expression tag
Lys 229	-	expression tag

B: 1 - 134 (UniProt: E3PPC4)

PDB	External Database	Details
Met -7	-	initiating methionine
Val -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Ala 40	Asp 76	conflict
Asp 41	Ala 77	conflict
Ala 42	Thr 78	conflict
Ala 55	Leu 91	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	41209.0
Non-Polymers*	Number of molecules	4
	Total formula weight	417.7
All*	Total formula weight	41626.8

*Water molecules are not included.