4XTR

Structure of Get3 bound to the transmembrane domain of Pep12

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	ATPase GET3	polymer	354	39392.6	2	UniProt (Q12154) Pfam (PF02374) In PDB	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)	Arsenical pump-driving ATPase, Arsenite-stimulated ATPase, Golgi to ER traffic protein 3, Guided entry of tail-anchored proteins 3
2	C, E	Antibody Heavy chain	polymer	230	24560.4	2		Homo sapiens synthetic construct
3	D, F	Antibody Light chain	polymer	217	23671.3	2		Homo sapiens synthetic construct
4	G	Pep12p	polymer	37	4575.7	1	UniProt (E7M086) In PDB	Saccharomyces cerevisiae (Baker's yeast)
5	A, B	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
6	A, B	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
7	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
8	A, B	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	2	Chemie (ATP)
9		water	water		18.0	928	Chemie (HOH)

Sequence modifications

A, B: 1 - 354 (UniProt: Q12154)

PDB	External Database	Details
Asn 57	Asp 57	engineered mutation

G: 262 - 288 (UniProt: E7M086)

PDB	External Database	Details
Met 252	-	initiating methionine
Gly 253	-	expression tag
Ser 254	-	expression tag
His 255	-	expression tag
His 256	-	expression tag
His 257	-	expression tag
His 258	-	expression tag
His 259	-	expression tag
His 260	-	expression tag
Ser 261	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	7
	Total formula weight	179824.3
Non-Polymers*	Number of molecules	7
	Total formula weight	1982.8
All*	Total formula weight	181807.1

*Water molecules are not included.