4XSZ
Crystal structure of CBR 9393 bound to Escherichia coli RNA polymerase holoenzyme
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, G, H | DNA-directed RNA polymerase subunit alpha | polymer | 239 | 26459.1 | 4 | UniProt (A7ZSI4) Pfam (PF01193) Pfam (PF01000) In PDB | Escherichia coli O139:H28 (strain E24377A / ETEC) | RNAP subunit alpha,RNA polymerase subunit alpha,Transcriptase subunit alpha |
| 2 | C, I | DNA-directed RNA polymerase subunit beta | polymer | 1342 | 150820.9 | 2 | UniProt (A7ZUK1) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) Pfam (PF04560) In PDB | Escherichia coli O139:H28 (strain E24377A / ETEC) | RNAP subunit beta,RNA polymerase subunit beta,Transcriptase subunit beta |
| 3 | D, J | DNA-directed RNA polymerase subunit beta' | polymer | 1407 | 155366.8 | 2 | UniProt (A7ZUK2) Pfam (PF04997) Pfam (PF00623) Pfam (PF04983) Pfam (PF05000) Pfam (PF04998) In PDB | Escherichia coli O139:H28 (strain E24377A / ETEC) | RNAP subunit beta',RNA polymerase subunit beta',Transcriptase subunit beta' |
| 4 | E, K | DNA-directed RNA polymerase subunit omega | polymer | 91 | 10249.5 | 2 | UniProt (B1IYV1) Pfam (PF01192) In PDB | Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) | RNAP omega subunit,RNA polymerase omega subunit,Transcriptase subunit omega |
| 5 | F, L | RNA polymerase sigma factor RpoD | polymer | 522 | 60315.3 | 2 | UniProt (P00579) Pfam (PF00140) Pfam (PF04546) Pfam (PF04542) Pfam (PF04539) Pfam (PF04545) In PDB | Escherichia coli (strain K12) | Sigma-70 |
| 6 | C, I | 4-[3-(4-fluorophenyl)-1H-pyrazol-4-yl]-N-[2-(piperazin-1-yl)ethyl]-2-(trifluoromethyl)aniline | non-polymer | 433.4 | 2 | Chemie (42U) | |||
| 7 | D, J | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 8 | D, J | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) |
Sequence modifications
A, B, G, H: 1 - 234 (UniProt: A7ZSI4)
| PDB | External Database | Details |
|---|---|---|
| Glu 235 | - | expression tag |
| Val 236 | - | expression tag |
| Leu 237 | - | expression tag |
| Phe 238 | - | expression tag |
| Gln 239 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 859341.4 | |
| Non-Polymers* | Number of molecules | 8 |
| Total formula weight | 1177.1 | |
| All* | Total formula weight | 860518.6 |
