4XSL

Crystal strcutre of D-tagatose 3-epimerase C66S from Pseudomonas cichorii in complex with glycerol

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	D-tagatose 3-epimerase	polymer	300	33858.6	4	UniProt (O50580) Pfam (PF01261)	Pseudomonas cichorii
2	E, J, N, S (A, B, C, D)	MANGANESE (II) ION	non-polymer		54.9	4	Chemie (MN)
3	F, G, H, I, K... (A, B, C, D)	GLYCEROL	non-polymer		92.1	14	Chemie (GOL)
4	W, X, Y, Z (A, B, C, D)	water	water		18.0	1268	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 290 (UniProt: O50580)

PDB	External Database	Details
Ser 66	Cys 66	engineered mutation
Gly 291	-	expression tag
Ser 292	-	expression tag
Arg 293	-	expression tag
Ser 294	-	expression tag
His 295	-	expression tag
His 296	-	expression tag
His 297	-	expression tag
His 298	-	expression tag
His 299	-	expression tag
His 300	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	135434.5
Non-Polymers*	Number of molecules	18
	Total formula weight	1509.1
All*	Total formula weight	136943.5

*Water molecules are not included.