4XL5

X-ray structure of bGFP-A / EGFP complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Green fluorescent protein	polymer	274	30641.3	1	UniProt (P42212) Pfam (PF01353)	Aequorea victoria (Jellyfish)
2	B (C)	bGFP-A	polymer	263	28694.7	1		synthetic construct
3	C, D (A, C)	water	water		18.0	103	Chemie (HOH)

Sequence modifications

A: 2 - 238 (UniProt: P42212)

PDB	External Database	Details
Met -20	-	initiating methionine
Arg -19	-	expression tag
Gly -18	-	expression tag
Ser -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Thr -10	-	expression tag
Asp -9	-	expression tag
Pro -8	-	expression tag
His -7	-	expression tag
Ala -6	-	expression tag
Ser -5	-	expression tag
Ser -4	-	expression tag
Ala -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
Pro 0	-	expression tag
Val 1	-	expression tag
Leu 64	Phe 64	engineered mutation
Cro 66	Ser 65	chromophore
Cro 66	Tyr 66	chromophore
Cro 66	Gly 67	chromophore
Leu 231	His 231	engineered mutation
Gly 239	-	expression tag
Ser 240	-	expression tag
Gly 241	-	expression tag
Ala 242	-	expression tag
Ser 243	-	expression tag
Glu 244	-	expression tag
Gln 245	-	expression tag
Lys 246	-	expression tag
Leu 247	-	expression tag
Ile 248	-	expression tag
Ser 249	-	expression tag
Glu 250	-	expression tag
Glu 251	-	expression tag
Asp 252	-	expression tag
Leu 253	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	59335.9
All*	Total formula weight	59335.9

*Water molecules are not included.