4WWL
E. coli 5'-nucleotidase mutant I521C labeled with MTSL (intermediate form)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Protein UshA | polymer | 533 | 59338.8 | 1 | UniProt (P07024) Pfam (PF00149) Pfam (PF02872) | Escherichia coli K-12 | 5'-nucleotidase |
| 2 | B (A) | S-[(1-oxyl-2,2,5,5-tetramethyl-2,5-dihydro-1H-pyrrol-3-yl)methyl] methanesulfonothioate | non-polymer | 264.4 | 1 | Chemie (MTN) | |||
| 3 | C, D (A) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 4 | E, F, G, H, I... (A) | SULFATE ION | non-polymer | 96.1 | 9 | Chemie (SO4) | |||
| 5 | N, O (A) | CARBONATE ION | non-polymer | 60.0 | 2 | Chemie (CO3) | |||
| 6 | P (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 7 | Q (A) | water | water | 18.0 | 427 | Chemie (HOH) |
Sequence modifications
A: 26 - 550 (UniProt: P07024)
| PDB | External Database | Details |
|---|---|---|
| Cys 521 | Ile 521 | engineered mutation |
| Leu 551 | - | expression tag |
| Glu 552 | - | expression tag |
| His 553 | - | expression tag |
| His 554 | - | expression tag |
| His 555 | - | expression tag |
| His 556 | - | expression tag |
| His 557 | - | expression tag |
| His 558 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 59338.8 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 1471.9 | |
| All* | Total formula weight | 60810.6 |
