4WVJ
Crystal structure of the Type-I signal peptidase from Staphylococcus aureus (SpsB) in complex with an inhibitor peptide (pep3).
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Maltose-binding periplasmic protein,Signal peptidase IB | polymer | 533 | 59189.8 | 1 | UniProt (P0AEY0) UniProt (Q5HHB9) Pfam (PF01547) Pfam (PF10502) UniProt (by SIFTS) (P0AEX9) In PDB | Escherichia coli K-12 | MBP,MMBP,Maltodextrin-binding protein,SPase IB,Leader peptidase IB |
2 | D | inhibitor peptide (PEP3) | polymer | 14 | 1157.3 | 1 | Staphylococcus aureus | ||
3 | B | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
4 | water | water | 18.0 | 374 | Chemie (HOH) |
Sequence modifications
A: 13 - 372 (UniProt: P0AEY0)
A: 377 - 526 (UniProt: Q5HHB9)
PDB | External Database | Details |
---|---|---|
Met 1 | - | initiating methionine |
Ser 2 | - | expression tag |
Tyr 3 | - | expression tag |
Tyr 4 | - | expression tag |
His 5 | - | expression tag |
His 6 | - | expression tag |
His 7 | - | expression tag |
His 8 | - | expression tag |
His 9 | - | expression tag |
His 10 | - | expression tag |
His 11 | - | expression tag |
Met 12 | - | expression tag |
Gln 28 | Glu 48 | SEE REMARK 999 |
Tyr 47 | Asp 67 | SEE REMARK 999 |
Cys 78 | Gln 98 | engineered mutation |
Gly 143 | Lys 163 | engineered mutation |
Asn 373 | Arg 393 | SEE REMARK 999 |
Ala 374 | - | linker |
Gly 375 | - | linker |
Ala 376 | - | linker |
PDB | External Database | Details |
---|---|---|
Ser 527 | - | expression tag |
His 528 | - | expression tag |
Pro 529 | - | expression tag |
Gln 530 | - | expression tag |
Phe 531 | - | expression tag |
Glu 532 | - | expression tag |
Lys 533 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 60347.1 | |
Branched | Number of molecules | 1 |
Total formula weight | 342.3 | |
All* | Total formula weight | 60689.4 |