4WVH

Crystal structure of the Type-I signal peptidase from Staphylococcus aureus (SpsB) in complex with a substrate peptide (pep1).

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Maltose-binding periplasmic protein,Signal peptidase IB	polymer	533	59189.8	1	UniProt (P0AEY0) UniProt (Q5HHB9) Pfam (PF01547) UniProt (by SIFTS) (P0AEX9) In PDB	Escherichia coli K-12	MBP,MMBP,Maltodextrin-binding protein,SPase IB,Leader peptidase IB
2	C	substrate peptide (pep1)	polymer	6	666.6	1		Staphylococcus aureus
3	B	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
4		water	water		18.0	246	Chemie (HOH)

Sequence modifications

A: 13 - 372 (UniProt: P0AEY0)

PDB	External Database	Details
Met 1	-	initiating methionine
Ser 2	-	expression tag
Tyr 3	-	expression tag
Tyr 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
His 11	-	expression tag
Met 12	-	expression tag
Gln 28	Glu 48	engineered mutation
Tyr 47	Asp 67	engineered mutation
Cys 78	Gln 98	engineered mutation
Gly 143	Lys 163	engineered mutation
Asn 373	-	linker
Ala 374	-	linker
Gly 375	-	linker
Ala 376	-	linker

A: 377 - 526 (UniProt: Q5HHB9)

PDB	External Database	Details
Ser 527	-	expression tag
His 528	-	expression tag
Pro 529	-	expression tag
Gln 530	-	expression tag
Phe 531	-	expression tag
Glu 532	-	expression tag
Lys 533	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	59856.4
Branched	Number of molecules	1
	Total formula weight	342.3
All*	Total formula weight	60198.7

*Water molecules are not included.