4WUO
Structure of the E270A Mutant Isopropylmalate dehydrogenase from Thermus thermophilus in complex with IPM, Mn and NADH
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | 3-isopropylmalate dehydrogenase | polymer | 359 | 38340.8 | 2 | UniProt (Q5SIY4) Pfam (PF00180) | Thermus thermophilus | 3-IPM-DH,Beta-IPM dehydrogenase,IMDH |
| 2 | C, Q (A) | 3-ISOPROPYLMALIC ACID | non-polymer | 176.2 | 2 | Chemie (IPM) | |||
| 3 | D, R (A, B) | NICOTINAMIDE-ADENINE-DINUCLEOTIDE | non-polymer | 663.4 | 2 | Chemie (NAD) | |||
| 4 | E, G (A) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
| 5 | F (A) | POTASSIUM ION | non-polymer | 39.1 | 1 | Chemie (K) | |||
| 6 | H, I, M, N, O... (A, B) | ETHANOL | non-polymer | 46.1 | 7 | Chemie (EOH) | |||
| 7 | J, K, L, P (A) | GLYCEROL | non-polymer | 92.1 | 4 | Chemie (GOL) | |||
| 8 | U, V (A, B) | water | water | 18.0 | 180 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 345 (UniProt: Q5SIY4)
| PDB | External Database | Details |
|---|---|---|
| Met -2 | - | initiating methionine |
| Ala -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Ala 270 | Glu 270 | engineered mutation |
| Ala 346 | - | expression tag |
| Ala 347 | - | expression tag |
| Ala 348 | - | expression tag |
| Leu 349 | - | expression tag |
| Glu 350 | - | expression tag |
| His 351 | - | expression tag |
| His 352 | - | expression tag |
| His 353 | - | expression tag |
| His 354 | - | expression tag |
| His 355 | - | expression tag |
| His 356 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 76681.7 | |
| Non-Polymers* | Number of molecules | 18 |
| Total formula weight | 2519.0 | |
| All* | Total formula weight | 79200.7 |
