4WTL
CRYSTAL STRUCTURE OF HCV NS5B GENOTYPE 2A JFH-1 ISOLATE WITH S15G E86Q E87Q C223H V321I MUTATIONS IN COMPLEX WITH RNA TEMPLATE 5'-UACC, RNA PRIMER 5'-PGG, MN2+, AND UDP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (T) | RNA TEMPLATE UACC | polymer | 4 | 1200.8 | 1 | synthetic construct | ||
| 2 | B (P) | RNA PRIMER GG | polymer | 2 | 645.5 | 1 | synthetic construct | ||
| 3 | C (A) | RNA-directed RNA polymerase | polymer | 580 | 64819.4 | 1 | UniProt (Q99IB8) | Hepatitis C virus JFH-1 | |
| 4 | D, E (A) | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) PubChem (27854) | |||
| 5 | F (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) PubChem (312) | |||
| 6 | G (A) | URIDINE-5'-DIPHOSPHATE | non-polymer | 404.2 | 1 | Chemie (UDP) PubChem (1158) PubChem (6031) PubChem (3084486) PubChem (6101632) PubChem (6604175) PubChem (11811538) PubChem (12049241) PubChem (25323044) PubChem (25323048) PubChem (44298965) PubChem (57448939) PubChem (53486216) PubChem (60098857) PubChem (91746160) PubChem (92332560) PubChem (92332561) PubChem (92332562) PubChem (92332563) PubChem (93088131) PubChem (93630083) | |||
| 7 | H (A) | 1-(2-METHOXY-ETHOXY)-2-{2-[2-(2-METHOXY-ETHOXY]-ETHOXY}-ETHANE | non-polymer | 266.3 | 1 | Chemie (PG6) PubChem (70931) | |||
| 8 | I (A) | 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 282.3 | 1 | Chemie (B3P) PubChem (125132) | |||
| 9 | J, K, L (T, P, A) | water | water | 18.0 | 481 | Chemie (HOH) |
Sequence modifications
A: 1 - 570 (UniProt: Q99IB8)
| PDB | External Database | Details |
|---|---|---|
| Met -1 | - | expression tag |
| Ser 0 | - | expression tag |
| Gly 15 | Ser 2457 | engineered mutation |
| Gln 86 | Glu 2528 | engineered mutation |
| Gln 87 | Glu 2529 | engineered mutation |
| His 223 | Cys 2665 | engineered mutation |
| Ile 321 | Val 2763 | engineered mutation |
| Leu 571 | - | expression tag |
| Glu 572 | - | expression tag |
| His 573 | - | expression tag |
| His 574 | - | expression tag |
| His 575 | - | expression tag |
| His 576 | - | expression tag |
| His 577 | - | expression tag |
| His 578 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 66665.6 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1098.2 | |
| All* | Total formula weight | 67763.8 |
