4WTG
CRYSTAL STRUCTURE OF HCV NS5B GENOTYPE 2A JFH-1 ISOLATE WITH S15G E86Q E87Q C223H V321I MUTATIONS AND DELTA8 BETA HAIRPIN LOOP DELETION IN COMPLEX WITH SOFOSBUVIR DIPHOSPHATE GS-607596, MN2+ AND SYMMETRICAL PRIMER TEMPLATE 5'-CAAAAUUU
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | T, P | RNA PRIMER TEMPLATE CAAAAUUU | polymer | 8 | 2495.5 | 2 | synthetic construct | ||
2 | A | RNA-directed RNA polymerase | polymer | 572 | 63755.2 | 1 | UniProt (Q99IB8) Pfam (PF00998) In PDB | Hepatitis C virus JFH-1 | |
3 | P, A | MANGANESE (II) ION | non-polymer | 54.9 | 3 | Chemie (MN) | |||
4 | P | 2'-deoxy-2'-fluoro-2'-methyluridine 5'-(trihydrogen diphosphate) | non-polymer | 420.2 | 1 | Chemie (6GS) | |||
5 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
6 | water | water | 18.0 | 59 | Chemie (HOH) |
Sequence modifications
A: 1 - 570 (UniProt: Q99IB8)
PDB | External Database | Details |
---|---|---|
Met -1 | - | expression tag |
Ser 0 | - | expression tag |
Gly 15 | Ser 2457 | engineered mutation |
Gln 86 | Glu 2528 | engineered mutation |
Gln 87 | Glu 2529 | engineered mutation |
His 223 | Cys 2665 | engineered mutation |
Ile 321 | Val 2763 | engineered mutation |
- | Asn 2886 | deletion |
- | Phe 2887 | deletion |
- | Glu 2888 | deletion |
- | Met 2889 | deletion |
- | Tyr 2890 | deletion |
- | Gly 2891 | deletion |
- | Ser 2892 | deletion |
- | Val 2893 | deletion |
Gly 444 | Tyr 2894 | linker |
Gly 445 | Ser 2895 | linker |
Leu 571 | - | expression tag |
Glu 572 | - | expression tag |
His 573 | - | expression tag |
His 574 | - | expression tag |
His 575 | - | expression tag |
His 576 | - | expression tag |
His 577 | - | expression tag |
His 578 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 68746.3 | |
Non-Polymers* | Number of molecules | 5 |
Total formula weight | 620.4 | |
All* | Total formula weight | 69366.7 |