4WPU
Crystal Structure of E83A mutant of Mtb PEPCK in complex with PEP and GDP
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Phosphoenolpyruvate carboxykinase [GTP] | polymer | 622 | 68988.7 | 1 | UniProt (A5TYT6) Pfam (PF17297) Pfam (PF00821) | Mycobacterium tuberculosis | PEPCK |
| 2 | B (A) | GUANOSINE-5'-DIPHOSPHATE | non-polymer | 443.2 | 1 | Chemie (GDP) PubChem (136073927) PubChem (135398619) PubChem (135402030) PubChem (135431654) PubChem (135566337) PubChem (135760556) PubChem (136026707) PubChem (136053418) PubChem (136663964) PubChem (136664748) PubChem (136759848) PubChem (136759849) PubChem (136759850) PubChem (136836636) PubChem (136847074) PubChem (172898537) | |||
| 3 | C (A) | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) PubChem (753) | |||
| 4 | D (A) | PHOSPHOENOLPYRUVATE | non-polymer | 168.0 | 1 | Chemie (PEP) PubChem (1005) | |||
| 5 | E (A) | water | water | 18.0 | 360 | Chemie (HOH) |
Sequence modifications
A: 1 - 606 (UniProt: A5TYT6)
| PDB | External Database | Details |
|---|---|---|
| His -15 | - | expression tag |
| His -14 | - | expression tag |
| His -13 | - | expression tag |
| His -12 | - | expression tag |
| His -11 | - | expression tag |
| His -10 | - | expression tag |
| Ser -9 | - | expression tag |
| Ser -8 | - | expression tag |
| Gly -7 | - | expression tag |
| Leu -6 | - | expression tag |
| Val -5 | - | expression tag |
| Pro -4 | - | expression tag |
| Ala -3 | - | expression tag |
| Gly -2 | - | expression tag |
| Ser -1 | - | expression tag |
| His 0 | - | expression tag |
| Ala 83 | Glu 83 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 68988.7 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 703.3 | |
| All* | Total formula weight | 69692.0 |
