4WPO

Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G in the pre-translocational state

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, HB (AA, CA)	23S Ribosomal RNA	polymer	2915	947895.4	2	GenBank (55771382)	Thermus thermophilus HB8
2	B, IB (AB, CB)	5S Ribosomal RNA	polymer	121	39188.4	2	GenBank (55771382)	Thermus thermophilus HB8
3	C, JB (AC, CC)	50S ribosomal protein L1	polymer	228	24741.5	2	UniProt (Q5SLP7) Pfam (PF00687)	Thermus thermophilus
4	D, KB (AD, CD)	50S ribosomal protein L2	polymer	276	30532.6	2	UniProt (P60405) Pfam (PF00181) Pfam (PF03947)	Thermus thermophilus
5	E, LB (AE, CE)	50S ribosomal protein L3	polymer	206	22450.2	2	UniProt (Q5SHN8) Pfam (PF00297)	Thermus thermophilus
6	F, MB (AF, CF)	50S ribosomal protein L4	polymer	210	23271.9	2	UniProt (Q5SHN9) Pfam (PF00573)	Thermus thermophilus	L1e
7	G, NB (AG, CG)	50S ribosomal protein L5	polymer	182	21061.6	2	UniProt (Q5SHQ0) Pfam (PF00281) Pfam (PF00673)	Thermus thermophilus
8	H, OB (AH, CH)	50S ribosomal protein L6	polymer	180	19568.9	2	UniProt (Q5SHQ3)	Thermus thermophilus
9	I, PB (AK, CK)	50S ribosomal protein L10	polymer	173	18586.6	2	UniProt (Q8VVE3) Pfam (PF00466)	Thermus thermophilus
10	J, QB (AL, CL)	50S ribosomal protein L11	polymer	147	15526.1	2	UniProt (Q5SLP6) Pfam (PF03946) Pfam (PF00298)	Thermus thermophilus
11	K, RB (AN, CN)	50S ribosomal protein L13	polymer	140	15927.9	2	UniProt (P60488) Pfam (PF00572)	Thermus thermophilus
12	L, SB (AO, CO)	50S ribosomal protein L14	polymer	122	13323.6	2	UniProt (Q5SHP8) Pfam (PF00238)	Thermus thermophilus
13	M, TB (AP, CP)	50S ribosomal protein L15	polymer	150	16319.1	2	UniProt (Q5SHQ7) Pfam (PF00828)	Thermus thermophilus
14	N, UB (AQ, CQ)	50S ribosomal protein L16	polymer	141	15993.9	2	UniProt (P60489) Pfam (PF00252)	Thermus thermophilus
15	O, VB (AR, CR)	50S ribosomal protein L17	polymer	118	13750.1	2	UniProt (Q9Z9H5) Pfam (PF01196)	Thermus thermophilus
16	P, WB (AS, CS)	50S ribosomal protein L18	polymer	112	12639.8	2	UniProt (Q5SHQ4) Pfam (PF00861)	Thermus thermophilus	TL24
17	Q, XB (AT, CT)	50S ribosomal protein L19	polymer	146	17188.8	2	UniProt (P60490) Pfam (PF01245)	Thermus thermophilus
18	R, YB (AU, CU)	50S ribosomal protein L20	polymer	118	13779.3	2	UniProt (P60491) Pfam (PF00453)	Thermus thermophilus
19	S, ZB (AV, CV)	50S ribosomal protein L21	polymer	101	11069.2	2	UniProt (P60492) Pfam (PF00829)	Thermus thermophilus
20	AC, T (AW, CW)	50S ribosomal protein L22	polymer	113	12808.1	2	UniProt (Q5SHP3) Pfam (PF00237)	Thermus thermophilus
21	BC, U (AX, CX)	50S ribosomal protein L23	polymer	96	10759.8	2	UniProt (Q5SHP0) Pfam (PF00276)	Thermus thermophilus
22	CC, V (AY, CY)	50S ribosomal protein L24	polymer	110	12085.6	2	UniProt (Q5SHP9) Pfam (PF00467) Pfam (PF17136)	Thermus thermophilus
23	DC, W (AZ, CZ)	50S ribosomal protein L25	polymer	206	23238.6	2	UniProt (Q5SHZ1) Pfam (PF01386) Pfam (PF14693)	Thermus thermophilus	TL5
24	EC, X (A0, C0)	50S ribosomal protein L27	polymer	85	9529.1	2	UniProt (P60493) Pfam (PF01016)	Thermus thermophilus
25	FC, Y (A1, C1)	50S ribosomal protein L28	polymer	98	11004.2	2	UniProt (P60494) Pfam (PF00830)	Thermus thermophilus
26	GC, Z (A2, C2)	50S ribosomal protein L29	polymer	72	8670.3	2	UniProt (Q5SHP6) Pfam (PF00831)	Thermus thermophilus
27	AA, HC (A3, C3)	50S ribosomal protein L30	polymer	60	6799.1	2	UniProt (Q5SHQ6) Pfam (PF00327)	Thermus thermophilus
28	BA, IC (A4, C4)	50S ribosomal protein L31	polymer	71	8300.5	2	UniProt (Q5SJE1) Pfam (PF01197)	Thermus thermophilus
29	CA, JC (A5, C5)	50S ribosomal protein L32	polymer	60	6722.1	2	UniProt (P80339) Pfam (PF01783)	Thermus thermophilus
30	DA, KC (A6, C6)	50S ribosomal protein L33	polymer	54	6632.9	2	UniProt (P35871) Pfam (PF00471)	Thermus thermophilus
31	EA, LC (A7, C7)	50S ribosomal protein L34	polymer	49	6132.4	2	UniProt (P80340) Pfam (PF00468)	Thermus thermophilus
32	FA, MC (A8, C8)	50S ribosomal protein L35	polymer	65	7506.2	2	UniProt (Q5SKU1) Pfam (PF01632)	Thermus thermophilus
33	GA, NC (A9, C9)	50S ribosomal protein L36	polymer	37	4435.4	2	UniProt (Q5SHR2) Pfam (PF00444)	Thermus thermophilus	Ribosomal protein B
34	HA, OC (BA, DA)	16S Ribosomal RNA	polymer	1521	493652.2	2	GenBank (55771382)	Thermus thermophilus HB8
35	IA, PC (BB, DB)	30S ribosomal protein S2	polymer	256	29317.7	2	UniProt (P80371) Pfam (PF00318)	Thermus thermophilus
36	JA, QC (BC, DC)	30S ribosomal protein S3	polymer	239	26751.1	2	UniProt (P80372) Pfam (PF07650) Pfam (PF00189)	Thermus thermophilus
37	KA, RC (BD, DD)	30S ribosomal protein S4	polymer	209	24373.4	2	UniProt (P80373) Pfam (PF00163) Pfam (PF01479)	Thermus thermophilus
38	LA, SC (BE, DE)	30S ribosomal protein S5	polymer	162	17583.4	2	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719)	Thermus thermophilus
39	MA, TC (BF, DF)	30S ribosomal protein S6	polymer	101	11988.8	2	UniProt (Q5SLP8) Pfam (PF01250)	Thermus thermophilus	TS9
40	NA, UC (BG, DG)	30S ribosomal protein S7	polymer	156	18051.0	2	UniProt (P17291) Pfam (PF00177)	Thermus thermophilus
41	OA, VC (BH, DH)	30S ribosomal protein S8	polymer	138	15868.6	2	UniProt (Q5SHQ2)	Thermus thermophilus
42	PA, WC (BI, DI)	30S ribosomal protein S9	polymer	128	14410.6	2	UniProt (P80374) Pfam (PF00380)	Thermus thermophilus
43	QA, XC (BJ, DJ)	30S ribosomal protein S10	polymer	105	11955.0	2	UniProt (Q5SHN7) Pfam (PF00338)	Thermus thermophilus
44	RA, YC (BK, DK)	30S ribosomal protein S11	polymer	129	13737.9	2	UniProt (P80376) Pfam (PF00411)	Thermus thermophilus
45	SA, ZC (BL, DL)	30S ribosomal protein S12	polymer	132	14637.4	2	UniProt (Q5SHN3) Pfam (PF00164)	Thermus thermophilus
46	AD, TA (BM, DM)	30S ribosomal protein S13	polymer	126	14338.9	2	UniProt (P80377) Pfam (PF00416)	Thermus thermophilus
47	BD, UA (BN, DN)	30S ribosomal protein S14 type Z	polymer	61	7158.7	2	UniProt (Q5SHQ1)	Thermus thermophilus
48	CD, VA (BO, DO)	30S ribosomal protein S15	polymer	89	10578.4	2	UniProt (Q5SJ76) Pfam (PF00312)	Thermus thermophilus
49	DD, WA (BP, DP)	30S ribosomal protein S16	polymer	88	10410.0	2	UniProt (Q5SJH3) Pfam (PF00886)	Thermus thermophilus
50	ED, XA (BQ, DQ)	30S ribosomal protein S17	polymer	105	12325.7	2	UniProt (Q5SHP7)	Thermus thermophilus
51	FD, YA (BR, DR)	30S ribosomal protein S18	polymer	88	10258.3	2	UniProt (Q5SLQ0) Pfam (PF01084)	Thermus thermophilus
52	GD, ZA (BS, DS)	30S ribosomal protein S19	polymer	93	10605.5	2	UniProt (Q5SHP2) Pfam (PF00203)	Thermus thermophilus
53	AB, HD (BT, DT)	30S ribosomal protein S20	polymer	106	11736.1	2	UniProt (P80380) Pfam (PF01649)	Thermus thermophilus
54	BB, ID (BU, DU)	30S ribosomal protein Thx	polymer	27	3350.0	2	UniProt (Q5SIH3) Pfam (PF17070)	Thermus thermophilus	S31
55	CB, JD (BV, DV)	mRNA	polymer	24	7804.7	2		synthetic construct
56	DB, KD (BW, DW)	A-site tRNA	polymer	76	24792.1	2	GenBank (557270520)	Escherichia coli str. K-12 substr. MC4100
57	EB, LD (BX, DX)	P-site tRNA	polymer	77	25005.1	2	GenBank (557270520)	Escherichia coli str. K-12 substr. MC4100
58	FB, MD (BY, DY)	E-site tRNA	polymer	76	24645.9	2	GenBank (557270520)	Escherichia coli str. K-12 substr. MC4100
59	GB, ND (BZ, DZ)	50S ribosomal protein L9,Elongation factor G	polymer	758	84416.8	2	UniProt (Q5SLQ1) UniProt (Q5SHN5) Pfam (PF01281) Pfam (PF00009) Pfam (PF03144) Pfam (PF14492) Pfam (PF03764) Pfam (PF00679)	Thermus thermophilus	EF-G
60	AAA, AAB, AAC, ABA, ABB... (AA, CA, DA, DK, AB...)	MAGNESIUM ION	non-polymer		24.3	2056	Chemie (MG)
61	VIA (AA)	POTASSIUM ION	non-polymer		39.1	1	Chemie (K)
62	ANA, AYB, BFC, CYB, DYB... (A9, C4, DN, C5, C6...)	ZINC ION	non-polymer		65.4	12	Chemie (ZN)
63	IVA, TEC (BD, DD)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
64	GFC, PWA (DZ, BZ)	GUANOSINE-5'-DIPHOSPHATE	non-polymer		443.2	2	Chemie (GDP)
65	AGC, AHC, BGC, BHC, CGC... (A0, CT, A1, CU, A3...)	water	water		18.0	3020	Chemie (HOH)

Sequence modifications

BZ, DZ: -66 - 7 (UniProt: Q5SLQ1)
BZ, DZ: 8 - 691 (UniProt: Q5SHN5)

Contents of the asymmetric unit

Polymers	Number of chains	118
	Total formula weight	4754384.7
Non-Polymers*	Number of molecules	2073
	Total formula weight	52384.8
All*	Total formula weight	4806769.5

*Water molecules are not included.