4WJV

Crystal structure of Rsa4 in complex with the Nsa2 binding peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D (A, B, C, D)	Ribosome assembly protein 4	polymer	381	42455.1	4	UniProt (P25382) Pfam (PF00400)	Saccharomyces cerevisiae (Baker's yeast)
2	E, F, G, H (E, F, G, H)	Maltose-binding periplasmic protein	polymer	381	41648.1	4	UniProt (P0AEX9) Pfam (PF01547)	Escherichia coli	MBP,MMBP,Maltodextrin-binding protein
3	I, J, K, L (I, J, K, L)	Ribosome biogenesis protein NSA2	polymer	22	2483.7	4	UniProt (P40078)	Saccharomyces cerevisiae (Baker's yeast)	NOP7-associated protein 2
4	M, N, O, P (M, N, O, P)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	4	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
5	Q, R, S, T, U... (A, B, C, D)	SULFATE ION	non-polymer		96.1	10	Chemie (SO4)

Sequence modifications

A, B, C, D: 137 - 515 (UniProt: P25382)

PDB	External Database	Details
Met 135	-	initiating methionine
Gly 136	-	expression tag

E, F, G, H: 11 - 377 (UniProt: P0AEX9)

PDB	External Database	Details
Met 1	-	initiating methionine
Lys 2	-	expression tag
His 3	-	expression tag
His 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
Pro 9	-	expression tag
Met 10	-	expression tag
Ala 92	Asp 108	engineered mutation
Ala 93	Lys 109	engineered mutation
Ala 249	Lys 265	engineered mutation
Ala 369	Glu 385	engineered mutation
Ala 372	Lys 388	engineered mutation
Ala 373	Asp 389	engineered mutation
Asn 377	Arg 393	engineered mutation
Ala 378	-	expression tag
Ala 379	-	expression tag
Ala 380	-	expression tag
Ala 381	-	expression tag

I, J, K, L: 80 - 101 (UniProt: P40078)

PDB	External Database	Details
Met 80	Leu 80	conflict

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	346347.7
Branched	Number of molecules	4
	Total formula weight	1369.2
Non-Polymers*	Number of molecules	10
	Total formula weight	960.6
All*	Total formula weight	348677.5

*Water molecules are not included.