4WJV
Crystal structure of Rsa4 in complex with the Nsa2 binding peptide
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Ribosome assembly protein 4 | polymer | 381 | 42455.1 | 4 | UniProt (P25382) Pfam (PF00400) | Saccharomyces cerevisiae (Baker's yeast) | |
| 2 | E, F, G, H (E, F, G, H) | Maltose-binding periplasmic protein | polymer | 381 | 41648.1 | 4 | UniProt (P0AEX9) Pfam (PF01547) | Escherichia coli | MBP,MMBP,Maltodextrin-binding protein |
| 3 | I, J, K, L (I, J, K, L) | Ribosome biogenesis protein NSA2 | polymer | 22 | 2483.7 | 4 | UniProt (P40078) | Saccharomyces cerevisiae (Baker's yeast) | NOP7-associated protein 2 |
| 4 | M, N, O, P (M, N, O, P) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 4 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 5 | Q, R, S, T, U... (A, B, C, D) | SULFATE ION | non-polymer | 96.1 | 10 | Chemie (SO4) |
Sequence modifications
A, B, C, D: 137 - 515 (UniProt: P25382)
E, F, G, H: 11 - 377 (UniProt: P0AEX9)
I, J, K, L: 80 - 101 (UniProt: P40078)
| PDB | External Database | Details |
|---|---|---|
| Met 135 | - | initiating methionine |
| Gly 136 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| Lys 2 | - | expression tag |
| His 3 | - | expression tag |
| His 4 | - | expression tag |
| His 5 | - | expression tag |
| His 6 | - | expression tag |
| His 7 | - | expression tag |
| His 8 | - | expression tag |
| Pro 9 | - | expression tag |
| Met 10 | - | expression tag |
| Ala 92 | Asp 108 | engineered mutation |
| Ala 93 | Lys 109 | engineered mutation |
| Ala 249 | Lys 265 | engineered mutation |
| Ala 369 | Glu 385 | engineered mutation |
| Ala 372 | Lys 388 | engineered mutation |
| Ala 373 | Asp 389 | engineered mutation |
| Asn 377 | Arg 393 | engineered mutation |
| Ala 378 | - | expression tag |
| Ala 379 | - | expression tag |
| Ala 380 | - | expression tag |
| Ala 381 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Met 80 | Leu 80 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 346347.7 | |
| Branched | Number of molecules | 4 |
| Total formula weight | 1369.2 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 960.6 | |
| All* | Total formula weight | 348677.5 |
