4WG2
P411BM3-CIS T438S I263F regioselective C-H amination catalyst
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C | Bifunctional P-450/NADPH-P450 reductase | polymer | 469 | 53611.1 | 3 | UniProt (P14779) Pfam (PF00067) In PDB | Bacillus megaterium | Cytochrome P450(BM-3),Cytochrome P450BM-3 |
2 | A, C | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
3 | A, B, C | PROTOPORPHYRIN IX CONTAINING FE | non-polymer | 616.5 | 3 | Chemie (HEM) | |||
4 | water | water | 18.0 | 66 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 463 (UniProt: P14779)
PDB | External Database | Details |
---|---|---|
Ala 78 | Val 79 | engineered mutation |
Val 87 | Phe 88 | engineered mutation |
Ser 142 | Pro 143 | engineered mutation |
Ile 175 | Thr 176 | engineered mutation |
Val 184 | Ala 185 | engineered mutation |
Arg 226 | Ser 227 | engineered mutation |
Gln 236 | His 237 | engineered mutation |
Gly 252 | Glu 253 | engineered mutation |
Phe 263 | Ile 264 | engineered mutation |
Ala 268 | Thr 269 | engineered mutation |
Val 290 | Ala 291 | engineered mutation |
Val 353 | Leu 354 | engineered mutation |
Val 366 | Ile 367 | engineered mutation |
Ser 400 | Cys 401 | engineered mutation |
Ser 438 | Thr 439 | engineered mutation |
Lys 442 | Glu 443 | engineered mutation |
His 464 | - | expression tag |
His 465 | - | expression tag |
His 466 | - | expression tag |
His 467 | - | expression tag |
His 468 | - | expression tag |
His 469 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 160833.2 | |
Non-Polymers* | Number of molecules | 6 |
Total formula weight | 2137.6 | |
All* | Total formula weight | 162970.8 |