4WFJ
Crystal structure of PET-degrading cutinase Cut190 S226P mutant in Ca(2+)-bound state at 1.75 angstrom resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Cutinase | polymer | 273 | 30287.9 | 1 | UniProt (W0TJ64) Pfam (PF03403) | Saccharomonospora viridis | |
| 2 | B (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 3 | C (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 4 | D (A) | water | water | 18.0 | 145 | Chemie (HOH) |
Sequence modifications
A: 47 - 304 (UniProt: W0TJ64)
| PDB | External Database | Details |
|---|---|---|
| Met 35 | - | expression tag |
| Arg 36 | - | expression tag |
| Gly 37 | - | expression tag |
| Ser 38 | - | expression tag |
| His 39 | - | expression tag |
| His 40 | - | expression tag |
| His 41 | - | expression tag |
| His 42 | - | expression tag |
| His 43 | - | expression tag |
| His 44 | - | expression tag |
| Gly 45 | - | expression tag |
| Ser 46 | - | expression tag |
| Pro 226 | Ser 226 | engineered mutation |
| Lys 305 | - | expression tag |
| Leu 306 | - | expression tag |
| Asn 307 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 30287.9 | |
| Non-Polymers* | Number of molecules | 2 |
| Total formula weight | 75.5 | |
| All* | Total formula weight | 30363.4 |
