4WFJ

Crystal structure of PET-degrading cutinase Cut190 S226P mutant in Ca(2+)-bound state at 1.75 angstrom resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Cutinase	polymer	273	30287.9	1	UniProt (W0TJ64) Pfam (PF03403) In PDB	Saccharomonospora viridis
2	A	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
3	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4		water	water		18.0	145	Chemie (HOH)

Sequence modifications

A: 47 - 304 (UniProt: W0TJ64)

PDB	External Database	Details
Met 35	-	expression tag
Arg 36	-	expression tag
Gly 37	-	expression tag
Ser 38	-	expression tag
His 39	-	expression tag
His 40	-	expression tag
His 41	-	expression tag
His 42	-	expression tag
His 43	-	expression tag
His 44	-	expression tag
Gly 45	-	expression tag
Ser 46	-	expression tag
Pro 226	Ser 226	engineered mutation
Lys 305	-	expression tag
Leu 306	-	expression tag
Asn 307	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30287.9
Non-Polymers*	Number of molecules	2
	Total formula weight	75.5
All*	Total formula weight	30363.4

*Water molecules are not included.