4WFI

Crystal structure of PET-degrading cutinase Cut190 S226P mutant in Ca(2+)-free state

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Cutinase	polymer	273	30287.9	1	UniProt (W0TJ64) Pfam (PF03403)	Saccharomonospora viridis
2	B (A)	water	water		18.0	226	Chemie (HOH)

Sequence modifications

A: 47 - 304 (UniProt: W0TJ64)

PDB	External Database	Details
Met 35	-	expression tag
Arg 36	-	expression tag
Gly 37	-	expression tag
Ser 38	-	expression tag
His 39	-	expression tag
His 40	-	expression tag
His 41	-	expression tag
His 42	-	expression tag
His 43	-	expression tag
His 44	-	expression tag
Gly 45	-	expression tag
Ser 46	-	expression tag
Pro 226	Ser 226	engineered mutation
Lys 305	-	expression tag
Leu 306	-	expression tag
Asn 307	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	30287.9
All*	Total formula weight	30287.9

*Water molecules are not included.