4WF0

Crystal Structure of iLID - an Improved Light-Inducible Dimer

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	NPH1-1	polymer	158	18287.6	2	UniProt (O49003) Pfam (PF13426)	Avena sativa (Oat)
2	C, D (A, B)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
3	E (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	F, G (A, B)	water	water		18.0	256	Chemie (HOH)

Sequence modifications

A, B: 404 - 543 (UniProt: O49003)

PDB	External Database	Details
Met 392	-	expression tag
Arg 393	-	expression tag
Gly 394	-	expression tag
Ser 395	-	expression tag
His 396	-	expression tag
His 397	-	expression tag
His 398	-	expression tag
His 399	-	expression tag
His 400	-	expression tag
His 401	-	expression tag
Gly 402	-	expression tag
Ser 403	-	expression tag
Val 493	Leu 493	engineered mutation
Tyr 502	Gln 502	engineered mutation
Arg 519	His 519	engineered mutation
Leu 520	Val 520	engineered mutation
His 521	Arg 521	engineered mutation
Gly 522	Asp 522	engineered mutation
Ala 528	Gly 528	engineered mutation
Cys 530	Met 530	engineered mutation
Phe 537	Glu 537	engineered mutation
Gln 538	Asn 538	engineered mutation
Ala 540	Asp 540	engineered mutation
Asn 544	-	expression tag
Asp 545	-	expression tag
Glu 546	-	expression tag
Asn 547	-	expression tag
Tyr 548	-	expression tag
Phe 549	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	36575.1
Non-Polymers*	Number of molecules	3
	Total formula weight	948.1
All*	Total formula weight	37523.3

*Water molecules are not included.