4WCV
Haloalkane dehalogenase DhaA mutant from Rhodococcus rhodochrous (T148L+G171Q+A172V+C176G)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Haloalkane dehalogenase | polymer | 299 | 34176.0 | 1 | UniProt (P0A3G3) Pfam (PF00561) UniProt (by SIFTS) (P0A3G2) | Rhodococcus rhodochrous | |
| 2 | B (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 3 | C (A) | TRIETHYLENE GLYCOL | non-polymer | 150.2 | 1 | Chemie (PGE) | |||
| 4 | D, E (A) | ACETATE ION | non-polymer | 59.0 | 2 | Chemie (ACT) | |||
| 5 | F (A) | water | water | 18.0 | 477 | Chemie (HOH) |
Sequence modifications
A: 1 - 293 (UniProt: P0A3G3)
| PDB | External Database | Details |
|---|---|---|
| Leu 148 | Thr 148 | engineered mutation |
| Gln 171 | Gly 171 | engineered mutation |
| Val 172 | Ala 172 | engineered mutation |
| Gly 176 | Cys 176 | engineered mutation |
| His 294 | - | expression tag |
| His 295 | - | expression tag |
| His 296 | - | expression tag |
| His 297 | - | expression tag |
| His 298 | - | expression tag |
| His 299 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 34176.0 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 303.7 | |
| All* | Total formula weight | 34479.7 |
