4W88
Crystal structure of XEG5A, a GH5 xyloglucan-specific endo-beta-1,4-glucanase from ruminal metagenomic library, in complex with a xyloglucan oligosaccharide and TRIS
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (B, A) | Xyloglucan-specific endo-beta-1,4-glucanase | polymer | 340 | 38423.8 | 2 | UniProt (D2K7Z0) | uncultured bacterium | |
| 2 | C (C) | beta-D-galactopyranose-(1-2)-alpha-D-xylopyranose-(1-6)-[beta-D-glucopyranose-(1-4)]beta-D-glucopyranose | branched | 636.6 | 1 | In PDB GlyTouCan (G23047PZ) | |||
| 3 | D (D) | alpha-D-xylopyranose-(1-6)-beta-D-glucopyranose-(1-4)-[beta-D-galactopyranose-(1-2)-alpha-D-xylopyranose-(1-6)]beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 930.8 | 1 | In PDB GlyTouCan (G23873IA) | |||
| 4 | E, G (B, A) | 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL | non-polymer | 122.1 | 2 | Chemie (TRS) | |||
| 5 | F, H (B, A) | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
| 6 | I, J (B, A) | water | water | 18.0 | 601 | Chemie (HOH) |
Sequence modifications
B, A: 92 - 430 (UniProt: D2K7Z0)
| PDB | External Database | Details |
|---|---|---|
| Thr 166 | Pro 166 | engineered mutation |
| Ala 431 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 76847.6 | |
| Branched | Number of molecules | 2 |
| Total formula weight | 1567.4 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 292.9 | |
| All* | Total formula weight | 78707.9 |
