4W57

T4 Lysozyme L99A with n-Butylbenzene Bound

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Endolysin	polymer	172	19691.5	1	UniProt (P00720) Pfam (PF00959)	Enterobacteria phage T4	Lysis protein,Lysozyme,Muramidase
2	B (A)	N-BUTYLBENZENE	non-polymer		134.2	1	Chemie (N4B)
3	C (A)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
4	D (A)	water	water		18.0	156	Chemie (HOH)

Sequence modifications

A: 1 - 164 (UniProt: P00720)

PDB	External Database	Details
Gly 12	Arg 12	variant
Ala 99	Leu 99	engineered mutation
Arg 137	Ile 137	variant
Leu 165	-	expression tag
Glu 166	-	expression tag
His 167	-	expression tag
His 168	-	expression tag
His 169	-	expression tag
His 170	-	expression tag
His 171	-	expression tag
His 172	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	19691.5
Non-Polymers*	Number of molecules	2
	Total formula weight	372.5
All*	Total formula weight	20064.1

*Water molecules are not included.