4V8B

Crystal structure analysis of ribosomal decoding (near-cognate tRNA-leu complex).

This is a non-PDB format compatible entry.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	AA, CA	16S ribosomal RNA	polymer	1506	489002.5	2	GenBank (AP008226.1)	Thermus thermophilus
2	AE, CE	30S RIBOSOMAL PROTEIN S2	polymer	256	29317.7	2	UniProt (P80371) Pfam (PF00318) In PDB	Thermus thermophilus
3	AF, CF	30S RIBOSOMAL PROTEIN S3	polymer	239	26751.1	2	UniProt (P80372) Pfam (PF07650) Pfam (PF00189) In PDB	Thermus thermophilus
4	AG, CG	30S RIBOSOMAL PROTEIN S4	polymer	208	24242.3	2	UniProt (P80373) Pfam (PF00163) Pfam (PF01479) In PDB	Thermus thermophilus
5	AH, CH	30S RIBOSOMAL PROTEIN S5	polymer	162	17583.4	2	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719) In PDB	Thermus thermophilus
6	AI, CI	30S RIBOSOMAL PROTEIN S6	polymer	101	11988.8	2	UniProt (Q5SLP8) Pfam (PF01250) In PDB	Thermus thermophilus
7	AJ, CJ	30S RIBOSOMAL PROTEIN S7	polymer	156	18051.0	2	UniProt (P17291) Pfam (PF00177) In PDB	Thermus thermophilus
8	AK, CK	30S RIBOSOMAL PROTEIN S8	polymer	138	15868.6	2	UniProt (Q5SHQ2) In PDB	Thermus thermophilus
9	AL, CL	30S RIBOSOMAL PROTEIN S9	polymer	128	14410.6	2	UniProt (P80374) Pfam (PF00380) In PDB	Thermus thermophilus
10	AM, CM	30S RIBOSOMAL PROTEIN S10	polymer	105	11955.0	2	UniProt (Q5SHN7) Pfam (PF00338) In PDB	Thermus thermophilus
11	AN, CN	30S RIBOSOMAL PROTEIN S11	polymer	129	13737.9	2	UniProt (P80376) Pfam (PF00411) In PDB	Thermus thermophilus
12	AO, CO	30S RIBOSOMAL PROTEIN S12	polymer	132	14637.4	2	UniProt (Q5SHN3) Pfam (PF00164) In PDB	Thermus thermophilus
13	AP, CP	30S RIBOSOMAL PROTEIN S13	polymer	126	14338.9	2	UniProt (P80377) Pfam (PF00416) In PDB	Thermus thermophilus
14	AQ, CQ	30S RIBOSOMAL PROTEIN S14	polymer	61	7158.7	2	UniProt (Q5SHQ1) In PDB	Thermus thermophilus
15	AR, CR	30S RIBOSOMAL PROTEIN S15	polymer	89	10578.4	2	UniProt (Q5SJ76) Pfam (PF00312) In PDB	Thermus thermophilus
16	AS, CS	30S RIBOSOMAL PROTEIN S16	polymer	88	10410.0	2	UniProt (Q5SJH3) Pfam (PF00886) In PDB	Thermus thermophilus
17	AT, CT	30S RIBOSOMAL PROTEIN S17	polymer	105	12325.7	2	UniProt (Q5SHP7) In PDB	Thermus thermophilus
18	AU, CU	30S RIBOSOMAL PROTEIN S18	polymer	88	10258.3	2	UniProt (Q5SLQ0) Pfam (PF01084) In PDB	Thermus thermophilus
19	AV, CV	30S RIBOSOMAL PROTEIN S19	polymer	93	10605.5	2	UniProt (Q5SHP2) Pfam (PF00203) In PDB	Thermus thermophilus
20	AW, CW	30S RIBOSOMAL PROTEIN S20	polymer	106	11736.1	2	UniProt (P80380) Pfam (PF01649) In PDB	Thermus thermophilus
21	AX, CX	30S RIBOSOMAL PROTEIN THX	polymer	27	3350.0	2	UniProt (Q5SIH3) Pfam (PF17070) In PDB	Thermus thermophilus
22	AB, CB	TRNA-LEU	polymer	87	28094.6	2	GenBank (AP012306.1)	Escherichia coli
23	AC, AD, CC, CD	TRNA-FMET	polymer	77	24801.8	4	GenBank (AP012306.1)	Escherichia coli
24	A1, C1	MRNA	polymer	10	3078.8	2	GenBank (AP012306.1)
25	BA, DA	RNA (2912-MER)	polymer	2912	947013.0	2	GenBank (AP008226.1)	Thermus thermophilus
26	BB, DB	5S RIBOSOMAL RNA	polymer	122	39540.6	2	GenBank (AP008226.1)	Thermus thermophilus
27	BD, DD	50S ribosomal protein L2	polymer	276	30532.6	2	UniProt (P60405) Pfam (PF00181) Pfam (PF03947) In PDB	Thermus thermophilus
28	BE, DE	50S ribosomal protein L3	polymer	206	22450.2	2	UniProt (Q5SHN8) Pfam (PF00297) In PDB	Thermus thermophilus
29	BF, DF	50S ribosomal protein L4	polymer	210	23271.9	2	UniProt (Q5SHN9) Pfam (PF00573) In PDB	Thermus thermophilus
30	BG, DG	50S ribosomal protein L5	polymer	182	21061.6	2	UniProt (Q5SHQ0) Pfam (PF00281) Pfam (PF00673) In PDB	Thermus thermophilus
31	BH, DH	50S ribosomal protein L6	polymer	180	19568.9	2	UniProt (Q5SHQ3) In PDB	Thermus thermophilus
32	BK, DK	50S ribosomal protein L9	polymer	148	16422.2	2	UniProt (Q5SLQ1) Pfam (PF01281) Pfam (PF03948) In PDB	Thermus thermophilus
33	BM, DM	50S ribosomal protein L13	polymer	140	15927.9	2	UniProt (P60488) Pfam (PF00572) In PDB	Thermus thermophilus
34	BN, DN	50S ribosomal protein L14	polymer	122	13323.6	2	UniProt (Q5SHP8) Pfam (PF00238) In PDB	Thermus thermophilus
35	BO, DO	50S ribosomal protein L15	polymer	150	16319.1	2	UniProt (Q5SHQ7) Pfam (PF00828) In PDB	Thermus thermophilus
36	BP, DP	50S ribosomal protein L16	polymer	141	15993.9	2	UniProt (P60489) Pfam (PF00252) In PDB	Thermus thermophilus
37	B0, D0	50S ribosomal protein L17	polymer	118	13750.1	2	UniProt (Q9Z9H5) Pfam (PF01196) In PDB	Thermus thermophilus
38	BQ, DQ	50S ribosomal protein L18	polymer	112	12639.8	2	UniProt (Q5SHQ4) Pfam (PF00861) In PDB	Thermus thermophilus
39	BR, DR	50S ribosomal protein L19	polymer	146	17188.8	2	UniProt (P60490) Pfam (PF01245) In PDB	Thermus thermophilus
40	B1, D1	50S ribosomal protein L20	polymer	118	13779.3	2	UniProt (P60491) Pfam (PF00453) In PDB	Thermus thermophilus
41	B2, D2	50S ribosomal protein L21	polymer	101	11069.2	2	UniProt (P60492) Pfam (PF00829) In PDB	Thermus thermophilus
42	BS, DS	50S ribosomal protein L22	polymer	113	12808.1	2	UniProt (Q5SHP3) Pfam (PF00237) In PDB	Thermus thermophilus
43	BT, DT	50S ribosomal protein L23	polymer	96	10759.8	2	UniProt (Q5SHP0) Pfam (PF00276) In PDB	Thermus thermophilus
44	BU, DU	50S ribosomal protein L24	polymer	110	12085.6	2	UniProt (Q5SHP9) Pfam (PF00467) Pfam (PF17136) In PDB	Thermus thermophilus
45	BV, DV	50S ribosomal protein L25	polymer	206	23238.6	2	UniProt (Q5SHZ1) Pfam (PF01386) Pfam (PF14693) In PDB	Thermus thermophilus
46	B3, D3	50S ribosomal protein L27	polymer	85	9529.1	2	UniProt (P60493) Pfam (PF01016) In PDB	Thermus thermophilus
47	BZ, DZ	50S ribosomal protein L28	polymer	98	11004.2	2	UniProt (P60494) Pfam (PF00830) In PDB	Thermus thermophilus
48	BW, DW	50S ribosomal protein L29	polymer	72	8670.3	2	UniProt (Q5SHP6) Pfam (PF00831) In PDB	Thermus thermophilus
49	BX, DX	50S ribosomal protein L30	polymer	60	6799.1	2	UniProt (Q5SHQ6) Pfam (PF00327) In PDB	Thermus thermophilus
50	B4, D4	50S ribosomal protein L31	polymer	71	8300.5	2	UniProt (Q5SJE1) Pfam (PF01197) In PDB	Thermus thermophilus
51	B5, D5	50S ribosomal protein L32	polymer	60	6722.1	2	UniProt (P80339) Pfam (PF01783) In PDB	Thermus thermophilus
52	B6, D6	50S ribosomal protein L33	polymer	54	6632.9	2	UniProt (P35871) Pfam (PF00471) In PDB	Thermus thermophilus
53	B7, D7	50S ribosomal protein L34	polymer	49	6132.4	2	UniProt (P80340) Pfam (PF00468) In PDB	Thermus thermophilus
54	B8, D8	50S ribosomal protein L35	polymer	65	7506.2	2	UniProt (Q5SKU1) Pfam (PF01632) In PDB	Thermus thermophilus
55	BA, DA, AA, BB, AC...	MAGNESIUM ION	non-polymer		24.3	1696	Chemie (MG)
56	AG, AQ, CG, CQ	ZINC ION	non-polymer		65.4	4	Chemie (ZN)

Sequence modifications

AC, AD, CC, CD: 1 - 77 (GenBank: AP012306.1)

PDB	External Database	Details
C 18	U 2457939	CONFLICT

BA, DA: 1 - 2902 (GenBank: AP008226.1)

PDB	External Database	Details
U 161	-	EXPRESSION TAG
A 654	G 352709	CONFLICT
C 654	G 352713	CONFLICT
G 654	C 352724	CONFLICT
A 654	C 352728	CONFLICT
U 1058	G 353133	CONFLICT
A 1080	C 353155	CONFLICT

Contents of the asymmetric unit

Polymers	Number of chains	110
	Total formula weight	4478252.6
Non-Polymers*	Number of molecules	1700
	Total formula weight	41482.9
All*	Total formula weight	4519735.5

*Water molecules are not included.