4V28
Structure of an E333Q variant of the GH99 endo-alpha-mannanase from Bacteroides xylanisolvens in complex with Man-Man-Methylumbelliferone
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | GLYCOSYL HYDROLASE FAMILY 71 | polymer | 380 | 43478.3 | 1 | UniProt (D6D1V7) Pfam (PF16317) | BACTEROIDES XYLANISOLVENS | ENDO-ALPHA-MANNANASE |
| 2 | B (B) | alpha-D-mannopyranose-(1-3)-alpha-D-mannopyranose | branched | 342.3 | 1 | In PDB BIRD (PRD_900112) GlyTouCan (G00891MP) | 3alpha-alpha-mannobiose | ||
| 3 | C (A) | 7-hydroxy-4-methyl-2H-chromen-2-one | non-polymer | 176.2 | 1 | Chemie (4MU) | |||
| 4 | D, F, G (A) | ACETATE ION | non-polymer | 59.0 | 3 | Chemie (ACT) | |||
| 5 | E (A) | 1,2-ETHANEDIOL | non-polymer | 62.1 | 1 | Chemie (EDO) | |||
| 6 | H (A) | water | water | 18.0 | 521 | Chemie (HOH) |
Sequence modifications
A: 1 - 380 (UniProt: D6D1V7)
| PDB | External Database | Details |
|---|---|---|
| Gln 333 | Gln 333 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 43478.3 | |
| Branched | Number of molecules | 1 |
| Total formula weight | 342.3 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 415.4 | |
| All* | Total formula weight | 44235.9 |
