4UUB

Crystal structure of zebrafish Sirtuin 5 in complex with 2R-butyl- succinylated CPS1-peptide

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	NAD-DEPENDENT PROTEIN DEACYLASE SIRTUIN-5, MITOCHONDRIAL	polymer	275	30423.8	2	UniProt (Q6DHI5) Pfam (PF02146)	DANIO RERIO (ZEBRAFISH)	SIRTUIN 5, REGULATORY PROTEIN SIR2 HOMOLOG 5
2	C (D)	CARBAMOYLPHOSPHATE SYNTHETASE I	polymer	9	969.1	1	UniProt (Q5R209) UniProt (by SIFTS) (P31327)	HOMO SAPIENS	2R-BUTYL-SUCCINYL-CPS1 PEPTIDE
3	D, F (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	E, J (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
5	G (B)	4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID	non-polymer		238.3	1	Chemie (EPE)
6	H (B)	DIMETHYL SULFOXIDE	non-polymer		78.1	1	Chemie (DMS)
7	I (B)	SODIUM ION	non-polymer		23.0	1	Chemie (NA)
8	K (D)	(2R)-2-butylbutanedioic acid	non-polymer		174.2	1	Chemie (SU8)
9	L, M (A, B)	water	water		18.0	58	Chemie (HOH)

Sequence modifications

A, B: 30 - 298 (UniProt: Q6DHI5)

PDB	External Database	Details
Gly 24	-	expression tag
Ile 25	-	expression tag
Asp 26	-	expression tag
Pro 27	-	expression tag
Phe 28	-	expression tag
Thr 29	-	expression tag

D: 1 - 8 (UniProt: Q5R209)

PDB	External Database	Details
Bez 0	-	modified residue

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	61816.7
Non-Polymers*	Number of molecules	8
	Total formula weight	768.6
All*	Total formula weight	62585.3

*Water molecules are not included.