4UQD

X-ray structure of glucuronoxylan-xylanohydrolase (Xyn30A) from Clostridium thermocellum at 1.25 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	CARBOHYDRATE BINDING FAMILY 6	polymer	409	46454.1	1	UniProt (E6UTM3) Pfam (PF17189) UniProt (by SIFTS) (A3DJS9)	RUMINICLOSTRIDIUM THERMOCELLUM	XYN30A
2	B, C (A)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL) PubChem (753)
3	D (A)	2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL	non-polymer		122.1	1	Chemie (TRS) PubChem (3777159)
4	E (A)	THREONINE	non-polymer		119.1	1	Chemie (THR) PubChem (17757249) PubChem (205) PubChem (69435) PubChem (90624) PubChem (99289) PubChem (6399258) PubChem (11768555) PubChem (17757244) PubChem (6288)
5	F (A)	water	water		18.0	638	Chemie (HOH)

Sequence modifications

A: 1 - 386 (UniProt: E6UTM3)

PDB	External Database	Details
Met -22	-	expression tag
Gly -21	-	expression tag
Ser -20	-	expression tag
Ser -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
Ser -12	-	expression tag
Ser -11	-	expression tag
Gly -10	-	expression tag
Leu -9	-	expression tag
Val -8	-	expression tag
Pro -7	-	expression tag
Arg -6	-	expression tag
Gly -5	-	expression tag
Ser -4	-	expression tag
His -3	-	expression tag
Met -2	-	expression tag
Ala -1	-	expression tag
Ser 0	-	expression tag
Ser 77	Phe 110	engineered mutation
Cys 315	Tyr 348	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	46454.1
Non-Polymers*	Number of molecules	4
	Total formula weight	425.5
All*	Total formula weight	46879.5

*Water molecules are not included.