4UOA

Structure of the A_Canine_Colorado_17864_06 H3 haemagglutinin Met29Ile mutant

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	HA1	polymer	327	36214.8	1	UniProt (E0UVR5) Pfam (PF00509)	INFLUENZA A VIRUS (A/CANINE/COLORADO/17864/2006(H3N8))	HEMAGGLUTININ, H3 HAEMAGGLUTININ HA1 CHAIN
2	B (B)	HA2	polymer	175	20259.3	1	UniProt (E0UVR5) Pfam (PF00509)	INFLUENZA A VIRUS (A/CANINE/COLORADO/17864/2006(H3N8))	HEMAGGLUTININ, H3 HAEMAGGLUTININ HA2 CHAIN
3	C (C)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	1	In PDB GlyTouCan (G42666HT)
4	D, E (D, E)	beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		586.5	2	In PDB GlyTouCan (G15407YE)
5	F, G (F, G)	alpha-D-mannopyranose-(1-6)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	2	In PDB GlyTouCan (G22573RC)
6	H (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
7	I, J, K, L (A, B)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
8	M, N (A, B)	water	water		18.0	40	Chemie (HOH)

Sequence modifications

A: 3 - 329 (UniProt: E0UVR5)

PDB	External Database	Details
-	Ile 20	deletion
Ile 29	Met 44	engineered mutation

B: 1 - 172 (UniProt: E0UVR5)

PDB	External Database	Details
Ser 173	-	expression tag
Gly 174	-	expression tag
Arg 175	-	expression tag
Glu 131	Asp 475	conflict
Val 161	Ile 505	conflict

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	56474.1
Branched	Number of molecules	5
	Total formula weight	3094.8
Non-Polymers*	Number of molecules	5
	Total formula weight	605.5
All*	Total formula weight	60174.4

*Water molecules are not included.