4UHB

Laboratory evolved variant R-C1 of potato epoxide hydrolase StEH1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	EPOXIDE HYDROLASE	polymer	328	37171.6	2	UniProt (Q41415) Pfam (PF00561)	SOLANUM TUBEROSUM (POTATO)	STEH1
2	C, D, E, F, H... (A, B)	GLYCEROL	non-polymer		92.1	9	Chemie (GOL)
3	G, M (A, B)	1,2-ETHANEDIOL	non-polymer		62.1	2	Chemie (EDO)
4	N, O (A, B)	water	water		18.0	591	Chemie (HOH)

Sequence modifications

A, B: 3 - 321 (UniProt: Q41415)

PDB	External Database	Details
Met 1	-	expression tag
Lys 2	-	expression tag
Thr 322	-	expression tag
Ser 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag
Lys 141	Val 141	engineered mutation
Val 155	Ile 155	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	74343.1
Non-Polymers*	Number of molecules	11
	Total formula weight	953.0
All*	Total formula weight	75296.1

*Water molecules are not included.