4UFN

Laboratory evolved variant R-C1B1 of potato epoxide hydrolase StEH1

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	EPOXIDE HYDROLASE	polymer	328	37164.6	2	UniProt (Q41415) Pfam (PF00561)	SOLANUM TUBEROSUM (POTATO)
2	C, D (A, B)	1,4-DIETHYLENE DIOXIDE	non-polymer		88.1	2	Chemie (DIO)
3	E, F (A, B)	water	water		18.0	642	Chemie (HOH)

Sequence modifications

A, B: 1 - 321 (UniProt: Q41415)

PDB	External Database	Details
Lys 2	Glu 2	cloning artifact
Leu 94	Pro 94	engineered mutation
Leu 106	Trp 106	engineered mutation
Tyr 109	Leu 109	engineered mutation
Lys 141	Val 141	engineered mutation
Val 155	Ile 155	engineered mutation
Thr 322	-	expression tag
Ser 323	-	expression tag
His 324	-	expression tag
His 325	-	expression tag
His 326	-	expression tag
His 327	-	expression tag
His 328	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	74329.1
Non-Polymers*	Number of molecules	2
	Total formula weight	176.2
All*	Total formula weight	74505.4

*Water molecules are not included.