4UEQ
Structure of the V74C large subunit mutant of D. fructosovorans NiFe- hydrogenase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | HYDROGENASE (NIFE) SMALL SUBUNIT HYDA | polymer | 264 | 28347.3 | 6 | UniProt (E1K248) Pfam (PF01058) Pfam (PF14720) | DESULFOVIBRIO FRUCTOSIVORANS JJ | NIFE-HYDROGENASE SMALL SUBUNIT |
| 2 | G, H, I, J, K... (Q, R, S, T, U...) | NICKEL-DEPENDENT HYDROGENASE LARGE SUBUNIT | polymer | 563 | 61220.9 | 6 | UniProt (E1K247) Pfam (PF00374) | DESULFOVIBRIO FRUCTOSIVORANS JJ | NIFE-HYDROGENASE LARGE SUBUNIT |
| 3 | AA, BA, DA, M, O... (E, F, A, B, C...) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 12 | Chemie (SF4) | |||
| 4 | CA, N, Q, T, W... (F, A, B, C, D...) | FE3-S4 CLUSTER | non-polymer | 295.8 | 6 | Chemie (F3S) | |||
| 5 | AB, EA, IB, MA, QB... (T, Q, U, R, V...) | CARBONMONOXIDE-(DICYANO) IRON | non-polymer | 135.9 | 6 | Chemie (FCO) | |||
| 6 | BB, FA, JB, NA, RB... (T, Q, U, R, V...) | NICKEL (II) ION | non-polymer | 58.7 | 6 | Chemie (NI) | |||
| 7 | CB, GA, KB, OA, SB... (T, Q, U, R, V...) | HYDROSULFURIC ACID | non-polymer | 34.1 | 6 | Chemie (H2S) | |||
| 8 | DB, FB, HA, JA, LB... (T, Q, U, R, V...) | MAGNESIUM ION | non-polymer | 24.3 | 10 | Chemie (MG) | |||
| 9 | EB, IA, MB, QA, UB... (T, Q, U, R, V...) | CALCIUM ION | non-polymer | 40.1 | 6 | Chemie (CA) | |||
| 10 | GB, HB, KA, LA, OB... (T, Q, U, R, V...) | GLYCEROL | non-polymer | 92.1 | 10 | Chemie (GOL) | |||
| 11 | WB (V) | CARBONATE ION | non-polymer | 60.0 | 1 | Chemie (CO3) | |||
| 12 | AC, BC, CC, DC, EC... (D, E, F, Q, R...) | water | water | 18.0 | 3410 | Chemie (HOH) |
Sequence modifications
Q, R, S, T, U, V: 2 - 549 (UniProt: E1K247)
| PDB | External Database | Details |
|---|---|---|
| Ala -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Trp -11 | - | expression tag |
| Ser -10 | - | expression tag |
| His -9 | - | expression tag |
| Pro -8 | - | expression tag |
| Gln -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Lys -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ala 1 | - | expression tag |
| Cys 74 | Val 74 | engineered mutation |
| Css 543 | Cys 543 | microheterogeneity |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 12 |
| Total formula weight | 537409.1 | |
| Non-Polymers* | Number of molecules | 63 |
| Total formula weight | 8830.9 | |
| All* | Total formula weight | 546240.0 |
