4UCQ
Structure of the T18D small subunit mutant of D. fructosovorans NiFe- hydrogenase
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | HYDROGENASE (NIFE) SMALL SUBUNIT HYDA | polymer | 264 | 28361.3 | 3 | UniProt (E1K248) Pfam (PF01058) Pfam (PF14720) UniProt (by SIFTS) (P18187) | DESULFOVIBRIO FRUCTOSIVORANS | NIFE-HYDROGENASE SMALL SUBUNIT |
| 2 | D, E, F (Q, R, S) | NICKEL-DEPENDENT HYDROGENASE LARGE SUBUNIT | polymer | 563 | 61200.8 | 3 | UniProt (E1K247) Pfam (PF00374) UniProt (by SIFTS) (P18188) | DESULFOVIBRIO FRUCTOSIVORANS | NIFE-HYDROGENASE LARGE SUBUNIT |
| 3 | G, I, K, M, N... (A, B, C) | IRON/SULFUR CLUSTER | non-polymer | 351.6 | 6 | Chemie (SF4) | |||
| 4 | H, L, O (A, B, C) | FE3-S4 CLUSTER | non-polymer | 295.8 | 3 | Chemie (F3S) | |||
| 5 | DA, J, T, U, Y... (S, A, Q, R) | GLYCEROL | non-polymer | 92.1 | 6 | Chemie (GOL) | |||
| 6 | AA, Q, V (S, Q, R) | CARBONMONOXIDE-(DICYANO) IRON | non-polymer | 135.9 | 3 | Chemie (FCO) | |||
| 7 | BA, R, W (S, Q, R) | NICKEL (II) ION | non-polymer | 58.7 | 3 | Chemie (NI) | |||
| 8 | CA, S, X (S, Q, R) | MAGNESIUM ION | non-polymer | 24.3 | 3 | Chemie (MG) | |||
| 9 | EA, FA, GA, HA, IA... (A, B, C, Q, R...) | water | water | 18.0 | 446 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 264 (UniProt: E1K248)
Q, R, S: 2 - 549 (UniProt: E1K247)
| PDB | External Database | Details |
|---|---|---|
| Asp 18 | Thr 68 | engineered mutation |
| PDB | External Database | Details |
|---|---|---|
| Ala -13 | - | expression tag |
| Ser -12 | - | expression tag |
| Trp -11 | - | expression tag |
| Ser -10 | - | expression tag |
| His -9 | - | expression tag |
| Pro -8 | - | expression tag |
| Gln -7 | - | expression tag |
| Phe -6 | - | expression tag |
| Glu -5 | - | expression tag |
| Lys -4 | - | expression tag |
| Gly -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Ser -1 | - | expression tag |
| Gly 0 | - | expression tag |
| Ala 1 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 268686.3 | |
| Non-Polymers* | Number of molecules | 24 |
| Total formula weight | 4206.5 | |
| All* | Total formula weight | 272892.7 |
