4U9K
Crystal structure of an H-NOX protein from S. oneidensis in the Mn(II)NO ligation state, Q154A/Q155A/K156A mutant
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | NO-binding heme-dependent sensor protein | polymer | 187 | 21157.2 | 2 | UniProt (Q8EF49) Pfam (PF07700) | Shewanella oneidensis | |
| 2 | C, G (A, B) | MANGANESE PROTOPORPHYRIN IX | non-polymer | 615.6 | 2 | Chemie (MNH) | |||
| 3 | D, H (A, B) | ZINC ION | non-polymer | 65.4 | 2 | Chemie (ZN) | |||
| 4 | E (A) | NITRIC OXIDE | non-polymer | 30.0 | 1 | Chemie (NO) | |||
| 5 | F (A) | SODIUM ION | non-polymer | 23.0 | 1 | Chemie (NA) | |||
| 6 | I, J (A, B) | water | water | 18.0 | 47 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 181 (UniProt: Q8EF49)
| PDB | External Database | Details |
|---|---|---|
| Ala 154 | Gln 154 | engineered mutation |
| Ala 155 | Gln 155 | engineered mutation |
| Ala 156 | Lys 156 | engineered mutation |
| Glu 182 | - | expression tag |
| Asn 183 | - | expression tag |
| Leu 184 | - | expression tag |
| Tyr 185 | - | expression tag |
| Phe 186 | - | expression tag |
| Gln 187 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 42314.5 | |
| Non-Polymers* | Number of molecules | 6 |
| Total formula weight | 1415.0 | |
| All* | Total formula weight | 43729.5 |
