4U95
Coupling of remote alternating-access transport mechanisms for protons and substrates in the multidrug efflux pump AcrB
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, B, C) | Multidrug efflux pump subunit AcrB | polymer | 1057 | 114678.2 | 3 | UniProt (P31224) Pfam (PF00873) | Escherichia coli | AcrAB-TolC multidrug efflux pump subunit AcrB,Acridine resistance protein B |
| 2 | D, E (D, E) | DARPin | polymer | 169 | 18317.6 | 2 | synthetic construct | ||
| 3 | F, G, I (A, B, C) | DODECYL-BETA-D-MALTOSIDE | non-polymer | 510.6 | 3 | Chemie (LMT) | |||
| 4 | H (B) | (4S,4AS,5AR,12AS)-4,7-BIS(DIMETHYLAMINO)-3,10,12,12A-TETRAHYDROXY-1,11-DIOXO-1,4,4A,5,5A,6,11,12A-OCTAHYDROTETRACENE-2- CARBOXAMIDE | non-polymer | 457.5 | 1 | Chemie (MIY) | |||
| 5 | J, K, L, M, N (A, B, C, D, E) | water | water | 18.0 | 2357 | Chemie (HOH) |
Sequence modifications
A, B, C: 1 - 1049 (UniProt: P31224)
| PDB | External Database | Details |
|---|---|---|
| Ala 940 | Lys 940 | engineered mutation |
| Leu 1050 | - | expression tag |
| Glu 1051 | - | expression tag |
| His 1052 | - | expression tag |
| His 1053 | - | expression tag |
| His 1054 | - | expression tag |
| His 1055 | - | expression tag |
| His 1056 | - | expression tag |
| His 1057 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 5 |
| Total formula weight | 380669.7 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 1989.3 | |
| All* | Total formula weight | 382659.0 |
