4U5K
Complex structure of mutant CtCel5E (E314A) with cellobiose
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Endoglucanase H | polymer | 403 | 46192.4 | 2 | UniProt (P16218) Pfam (PF00150) In PDB | Clostridium thermocellum ATCC 27405 | Cellulase H,Endo-1,4-beta-glucanase H,EgH, CEL5E |
2 | C, D | beta-D-glucopyranose-(1-4)-beta-D-glucopyranose | branched | 342.3 | 2 | In PDB BIRD (PRD_900005) GlyTouCan (G84824ZO) | beta-cellobiose | ||
3 | water | water | 18.0 | 524 | Chemie (HOH) |
Sequence modifications
A, B: 39 - 403 (UniProt: P16218)
PDB | External Database | Details |
---|---|---|
Met 1 | - | expression tag |
Gly 2 | - | expression tag |
Ser 3 | - | expression tag |
Ser 4 | - | expression tag |
His 5 | - | expression tag |
His 6 | - | expression tag |
His 7 | - | expression tag |
His 8 | - | expression tag |
His 9 | - | expression tag |
His 10 | - | expression tag |
Ser 11 | - | expression tag |
Ser 12 | - | expression tag |
Gly 13 | - | expression tag |
Leu 14 | - | expression tag |
Val 15 | - | expression tag |
Pro 16 | - | expression tag |
Arg 17 | - | expression tag |
Gly 18 | - | expression tag |
Ser 19 | - | expression tag |
His 20 | - | expression tag |
Met 21 | - | expression tag |
Ala 22 | - | expression tag |
Ser 23 | - | expression tag |
Met 24 | - | expression tag |
Thr 25 | - | expression tag |
Gly 26 | - | expression tag |
Gly 27 | - | expression tag |
Gln 28 | - | expression tag |
Gln 29 | - | expression tag |
Met 30 | - | expression tag |
Gly 31 | - | expression tag |
Arg 32 | - | expression tag |
Ile 33 | - | expression tag |
Glu 34 | - | expression tag |
Gly 35 | - | expression tag |
Arg 36 | - | expression tag |
Glu 37 | - | expression tag |
Phe 38 | - | expression tag |
Ala 314 | Glu 565 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 92384.8 | |
Branched | Number of molecules | 2 |
Total formula weight | 684.6 | |
All* | Total formula weight | 93069.4 |