4U2E
Crystal structure of dienelactone hydrolase S-3 variant (Q35H, F38L, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G, G211D and K234N) at 1.70 A resolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Carboxymethylenebutenolidase | polymer | 236 | 25192.5 | 1 | UniProt (P0A115) Pfam (PF01738) | Pseudomonas sp. | Dienelactone hydrolase,DLH |
| 2 | B, C, D (A) | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
| 3 | E (A) | water | water | 18.0 | 172 | Chemie (HOH) |
Sequence modifications
A: 1 - 236 (UniProt: P0A115)
| PDB | External Database | Details |
|---|---|---|
| His 35 | Gln 35 | engineered mutation |
| Leu 38 | Phe 38 | engineered mutation |
| Ala 79 | Arg 79 | conflict |
| Leu 110 | Gln 110 | engineered mutation |
| Ser 123 | Cys 123 | engineered mutation |
| Cys 137 | Tyr 137 | engineered mutation |
| Cys 145 | Tyr 145 | engineered mutation |
| Asp 154 | Lys 154 | engineered mutation |
| Gly 199 | Glu 199 | engineered mutation |
| Gly 208 | Ser 208 | engineered mutation |
| Asp 211 | Gly 211 | engineered mutation |
| Thr 224 | Arg 224 | conflict |
| Asn 234 | Lys 234 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 25192.5 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 288.2 | |
| All* | Total formula weight | 25480.6 |
