4U2D

Crystal structure of dienelactone hydrolase S-2 variant (Q35H, F38L, Q110L, C123S, Y137C, Y145C, N154D, E199G, S208G and G211D) at 1.67 A resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Carboxymethylenebutenolidase	polymer	236	25207.5	1	UniProt (P0A115) Pfam (PF01738) In PDB	Pseudomonas sp.	Dienelactone hydrolase,DLH
2	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3		water	water		18.0	200	Chemie (HOH)

Sequence modifications

A: 1 - 236 (UniProt: P0A115)

PDB	External Database	Details
His 35	Gln 35	engineered mutation
Leu 38	Phe 38	engineered mutation
Ala 79	Arg 79	conflict
Leu 110	Gln 110	engineered mutation
Ser 123	Cys 123	engineered mutation
Cys 137	Tyr 137	engineered mutation
Cys 145	Tyr 145	engineered mutation
Asp 154	Lys 154	engineered mutation
Gly 199	Glu 199	engineered mutation
Gly 208	Ser 208	engineered mutation
Asp 211	Gly 211	engineered mutation
Thr 224	Arg 224	conflict

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	25207.5
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	25399.7

*Water molecules are not included.