4U15

M3-mT4L receptor bound to tiotropium

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Muscarinic acetylcholine receptor M3,Lysozyme,Muscarinic acetylcholine receptor M3	polymer	418	47858.0	2	UniProt (P08483) UniProt (D9IEF7) Pfam (PF00001) Pfam (PF00959)	Rattus norvegicus (Rat)
2	C, I (A, B)	(1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane	non-polymer		392.5	2	Chemie (0HK)
3	D, E, F, L (A, B)	HEXAETHYLENE GLYCOL	non-polymer		282.3	4	Chemie (P6G)
4	G, H, M, N (A, B)	D(-)-TARTARIC ACID	non-polymer		150.1	4	Chemie (TAR)
5	J, K (B)	(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate	non-polymer		356.5	2	Chemie (OLC)
6	O (A)	water	water		18.0	1	Chemie (HOH)

Sequence modifications

A, B: 57 - 259 (UniProt: P08483)

PDB	External Database	Details
Gly 56	-	expression tag

A, B: 1018 - 1118 (UniProt: D9IEF7)

PDB	External Database	Details
Ala 1054	Cys 97	conflict

A, B: 482 - 563 (UniProt: P08483)

PDB	External Database	Details
Met 1001	-	linker
Asn 1002	-	linker
Ile 1003	-	linker
Phe 1004	-	linker
Glu 1005	-	linker
Met 1006	-	linker
Leu 1007	-	linker
Arg 1008	-	linker
Ile 1009	-	linker
Asp 1010	-	linker
Glu 1011	-	linker
Gly 1012	-	linker
Gly 1013	-	linker
Gly 1014	-	linker
Ser 1015	-	linker
Gly 1016	-	linker
Gly 1017	-	linker
Lys 564	-	expression tag
Arg 565	-	expression tag
Lys 566	-	expression tag
Arg 567	-	expression tag
Arg 568	-	expression tag
Lys 569	-	expression tag
His 570	-	expression tag
His 571	-	expression tag
His 572	-	expression tag
His 573	-	expression tag
His 574	-	expression tag
His 575	-	expression tag
His 576	-	expression tag
His 577	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	95716.0
Non-Polymers*	Number of molecules	12
	Total formula weight	3227.8
All*	Total formula weight	98943.8

*Water molecules are not included.