4U14

Structure of the M3 muscarinic acetylcholine receptor bound to the antagonist tiotropium crystallized with disulfide-stabilized T4 lysozyme (dsT4L)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Muscarinic acetylcholine receptor M3,Endolysin,Muscarinic acetylcholine receptor M3	polymer	460	52684.8	1	UniProt (P08483) UniProt (P00720) Pfam (PF00001) Pfam (PF00959)	Rattus norvegicus (Rat)	Lysis protein,Lysozyme,Muramidase
2	B (A)	(1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane	non-polymer		392.5	1	Chemie (0HK) PubChem (60831) PubChem (131951) PubChem (50909376) PubChem (123134303) PubChem (58667495) PubChem (59084585) PubChem (59168855) PubChem (59288009) PubChem (60003260) PubChem (87909275) PubChem (98047845) PubChem (122130800) PubChem (124527228) PubChem (656611) PubChem (3086655) PubChem (5487427) PubChem (5748851) PubChem (9804844) PubChem (11598033) PubChem (15057856)

Sequence modifications

A: 57 - 259 (UniProt: P08483)

PDB	External Database	Details
Gly 56	-	expression tag

A: 1001 - 1161 (UniProt: P00720)

PDB	External Database	Details
Cys 1003	Ile 3	conflict
Gly 1012	Arg 12	conflict
Cys 1021	Thr 21	conflict
Thr 1054	Cys 54	conflict
Arg 1137	Ile 137	conflict
Cys 1142	Thr 142	conflict

A: 482 - 563 (UniProt: P08483)

PDB	External Database	Details
Lys 564	-	expression tag
Arg 565	-	expression tag
Lys 566	-	expression tag
Arg 567	-	expression tag
Arg 568	-	expression tag
Lys 569	-	expression tag
His 570	-	expression tag
His 571	-	expression tag
His 572	-	expression tag
His 573	-	expression tag
His 574	-	expression tag
His 575	-	expression tag
His 576	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	52684.8
Non-Polymers*	Number of molecules	1
	Total formula weight	392.5
All*	Total formula weight	53077.3

*Water molecules are not included.