4U14

Structure of the M3 muscarinic acetylcholine receptor bound to the antagonist tiotropium crystallized with disulfide-stabilized T4 lysozyme (dsT4L)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Muscarinic acetylcholine receptor M3,Endolysin,Muscarinic acetylcholine receptor M3	polymer	460	52684.8	1	UniProt (P08483) UniProt (P00720) Pfam (PF00001) Pfam (PF00959)	Rattus norvegicus (Rat)	Lysis protein,Lysozyme,Muramidase
2	B (A)	(1R,2R,4S,5S,7S)-7-{[hydroxy(dithiophen-2-yl)acetyl]oxy}-9,9-dimethyl-3-oxa-9-azoniatricyclo[3.3.1.0~2,4~]nonane	non-polymer		392.5	1	Chemie (0HK)

Sequence modifications

A: 57 - 259 (UniProt: P08483)

PDB	External Database	Details
Gly 56	-	expression tag

A: 1001 - 1161 (UniProt: P00720)

PDB	External Database	Details
Cys 1003	Ile 3	conflict
Gly 1012	Arg 12	conflict
Cys 1021	Thr 21	conflict
Thr 1054	Cys 54	conflict
Arg 1137	Ile 137	conflict
Cys 1142	Thr 142	conflict

A: 482 - 563 (UniProt: P08483)

PDB	External Database	Details
Lys 564	-	expression tag
Arg 565	-	expression tag
Lys 566	-	expression tag
Arg 567	-	expression tag
Arg 568	-	expression tag
Lys 569	-	expression tag
His 570	-	expression tag
His 571	-	expression tag
His 572	-	expression tag
His 573	-	expression tag
His 574	-	expression tag
His 575	-	expression tag
His 576	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	52684.8
Non-Polymers*	Number of molecules	1
	Total formula weight	392.5
All*	Total formula weight	53077.3

*Water molecules are not included.