4TT3

The Pathway of Binding of the Intrinsically Disordered Mitochondrial Inhibitor Protein to F1-ATPase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	ATP synthase subunit alpha, mitochondrial	polymer	510	55301.2	3	UniProt (P19483) Pfam (PF02874) Pfam (PF00006) Pfam (PF00306)	Bos taurus (Bovine)
2	D, E, F (D, E, F)	ATP synthase subunit beta, mitochondrial	polymer	480	51615.7	3	UniProt (P00829) Pfam (PF02874) Pfam (PF00006) Pfam (PF22919)	Bos taurus (Bovine)
3	G (G)	ATP synthase subunit gamma, mitochondrial	polymer	273	30300.8	1	UniProt (P05631) Pfam (PF00231)	Bos taurus (Bovine)	F-ATPase gamma subunit
4	H, I, J (H, I, J)	ATPase inhibitor, mitochondrial	polymer	66	7404.0	3	UniProt (P01096) Pfam (PF04568)	Bos taurus (Bovine)	Inhibitor of F(1)F(o)-ATPase,IF1
5	K, N, Q (A, B, C)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	3	Chemie (ATP)
6	L, O, R, T, V (A, B, C, D, F)	MAGNESIUM ION	non-polymer		24.3	5	Chemie (MG)
7	M, P (A, B)	GLYCEROL	non-polymer		92.1	2	Chemie (GOL)
8	S, U (D, F)	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	2	Chemie (ADP)
9	AA, BA, W, X, Y... (E, F, A, B, C...)	water	water		18.0	28	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 510 (UniProt: P19483)

PDB	External Database	Details
Gly 481	Ser 524	see sequence details

H, I, J: 1 - 60 (UniProt: P01096)

PDB	External Database	Details
Ala 39	Lys 64	engineered mutation
His 61	-	expression tag
His 62	-	expression tag
His 63	-	expression tag
His 64	-	expression tag
His 65	-	expression tag
His 66	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	373263.4
Non-Polymers*	Number of molecules	12
	Total formula weight	2681.7
All*	Total formula weight	375945.1

*Water molecules are not included.