4S20

Structural basis for transcription reactivation by RapA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, F, G (A, B, F, G)	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	4	UniProt (B1X6E7) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4)	Escherichia coli	RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha
2	C, H (C, H)	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	2	UniProt (K0AVA1) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2)	Escherichia coli	RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta
3	D, I (D, I)	DNA-directed RNA polymerase subunit beta'	polymer	1416	156537.0	2	UniProt (K0BCS5)	Escherichia coli	RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta'
4	E, J (E, J)	DNA-directed RNA polymerase subunit omega	polymer	90	10118.4	2	UniProt (K0BU55)	Escherichia coli	RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega
5	K, L (K, L)	RNA polymerase-associated protein RapA	polymer	974	110672.7	2	UniProt (P60240) Pfam (PF18339) Pfam (PF18337) Pfam (PF00176) Pfam (PF00271) Pfam (PF12137)	Escherichia coli	ATP-dependent helicase HepA
6	M, O (M, O)	5'-D(P*AP*CP*GP*AP*CP*TP*GP*AP*GP*CP*CP*GP*AP*TP*G)-3'	polymer	15	4619.0	2
7	N, P (N, P)	5'-R(P*AP*UP*CP*GP*GP*CP*UP*CP*A)-3'	polymer	9	2831.7	2
8	Q, R, T, U (D, I)	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
9	S, V (D, I)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

D, I: 1 - 1407 (UniProt: K0BCS5)

PDB	External Database	Details
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Val 1410	-	expression tag
His 1411	-	expression tag
His 1412	-	expression tag
His 1413	-	expression tag
His 1414	-	expression tag
His 1415	-	expression tag
His 1416	-	expression tag

K, L: 1 - 968 (UniProt: P60240)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Cys 350	Arg 350	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	1017434.2
Non-Polymers*	Number of molecules	6
	Total formula weight	310.2
All*	Total formula weight	1017744.5

*Water molecules are not included.