4S20

Structural basis for transcription reactivation by RapA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, F, G	DNA-directed RNA polymerase subunit alpha	polymer	329	36558.7	4	UniProt (B1X6E7) Pfam (PF01193) Pfam (PF01000) Pfam (PF03118) UniProt (by SIFTS) (P0A7Z4) In PDB	Escherichia coli	RNAP subunit alpha, RNA polymerase subunit alpha, Transcriptase subunit alpha
2	C, H	DNA-directed RNA polymerase subunit beta	polymer	1342	150820.9	2	UniProt (K0AVA1) Pfam (PF04560) Pfam (PF04563) Pfam (PF04561) Pfam (PF04565) Pfam (PF10385) Pfam (PF00562) UniProt (by SIFTS) (P0A8V2) In PDB	Escherichia coli	RNAP subunit beta, RNA polymerase subunit beta, Transcriptase subunit beta
3	D, I	DNA-directed RNA polymerase subunit beta'	polymer	1416	156537.0	2	UniProt (K0BCS5) In PDB	Escherichia coli	RNAP subunit beta', RNA polymerase subunit beta', Transcriptase subunit beta'
4	E, J	DNA-directed RNA polymerase subunit omega	polymer	90	10118.4	2	UniProt (K0BU55) In PDB	Escherichia coli	RNAP omega subunit, RNA polymerase omega subunit, Transcriptase subunit omega
5	K, L	RNA polymerase-associated protein RapA	polymer	974	110672.7	2	UniProt (P60240) Pfam (PF18339) Pfam (PF18337) Pfam (PF00176) Pfam (PF00271) Pfam (PF12137) In PDB	Escherichia coli	ATP-dependent helicase HepA
6	M, O	5'-D(P*AP*CP*GP*AP*CP*TP*GP*AP*GP*CP*CP*GP*AP*TP*G)-3'	polymer	15	4619.0	2
7	N, P	5'-R(P*AP*UP*CP*GP*GP*CP*UP*CP*A)-3'	polymer	9	2831.7	2
8	D, I	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
9	D, I	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)

Sequence modifications

D, I: 1 - 1407 (UniProt: K0BCS5)

PDB	External Database	Details
Leu 1408	-	expression tag
Glu 1409	-	expression tag
Val 1410	-	expression tag
His 1411	-	expression tag
His 1412	-	expression tag
His 1413	-	expression tag
His 1414	-	expression tag
His 1415	-	expression tag
His 1416	-	expression tag

K, L: 1 - 968 (UniProt: P60240)

PDB	External Database	Details
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag
Cys 350	Arg 350	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	16
	Total formula weight	1017434.2
Non-Polymers*	Number of molecules	6
	Total formula weight	310.2
All*	Total formula weight	1017744.5

*Water molecules are not included.