4S04

Crystal structure of Klebsiella pneumoniae PmrA in complex with PmrA box DNA

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F (A, B, E, F)	DNA-binding transcriptional regulator BasR	polymer	232	26305.9	4	UniProt (S5YJU7) Pfam (PF00072) Pfam (PF00486) UniProt (by SIFTS) (A0A0R4I965)	Klebsiella pneumoniae
2	C, G (C, G)	DNA (25-MER)	polymer	25	7631.0	2		synthetic construct
3	D, H (D, H)	DNA (25-MER)	polymer	25	7720.0	2		synthetic construct
4	I, K, M, O (A, B, E, F)	BERYLLIUM TRIFLUORIDE ION	non-polymer		66.0	4	Chemie (BEF)
5	J, L, N, P (A, B, E, F)	MAGNESIUM ION	non-polymer		24.3	4	Chemie (MG)
6	Q, R, S, T, U... (A, B, C, D, E...)	water	water		18.0	86	Chemie (HOH)

Sequence modifications

A, B, E, F: 1 - 223 (UniProt: S5YJU7)

PDB	External Database	Details
Gly 181	Trp 181	engineered mutation
Asp 220	Ile 220	engineered mutation
His 224	-	expression tag
Leu 225	-	expression tag
Glu 226	-	expression tag
His 227	-	expression tag
His 228	-	expression tag
His 229	-	expression tag
His 230	-	expression tag
His 231	-	expression tag
His 232	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	135925.7
Non-Polymers*	Number of molecules	8
	Total formula weight	361.2
All*	Total formula weight	136287.0

*Water molecules are not included.