4RX3
A triple mutant in the omega-loop of TEM-1 beta-lactamase changes the substrate profile via a large conformational change and an altered general base for catalysis
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Beta-lactamase TEM | polymer | 263 | 28882.9 | 1 | UniProt (P62593) Pfam (PF00144) Pfam (PF13354) In PDB | Escherichia coli | IRT-4, Penicillinase, TEM-1, TEM-16/CAZ-7, TEM-2, TEM-24/CAZ-6, TEM-3, TEM-4, TEM-5, TEM-6, TEM-8/CAZ-2 |
| 2 | A | CITRATE ANION | non-polymer | 189.1 | 1 | Chemie (FLC) | |||
| 3 | water | water | 18.0 | 430 | Chemie (HOH) |
Sequence modifications
A: 26 - 290 (UniProt: P62593)
| PDB | External Database | Details |
|---|---|---|
| Gly 70 | Ser 68 | ENGINEERED MUTATION |
| Tyr 165 | Trp 163 | ENGINEERED MUTATION |
| Tyr 166 | Glu 164 | ENGINEERED MUTATION |
| Gly 167 | Pro 165 | ENGINEERED MUTATION |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 28882.9 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 189.1 | |
| All* | Total formula weight | 29072.0 |
